Skip to main content

ORIGINAL RESEARCH article

Front. Cell. Infect. Microbiol.
Sec. Biofilms
Volume 14 - 2024 | doi: 10.3389/fcimb.2024.1426791
This article is part of the Research Topic Vaginal Dysbiosis and Biofilms: Volume II View all 9 articles

Synergistic In Vitro Activity and Mechanism of KBN Lotion and Miconazole Nitrate Against Drug-Resistant Candida albicans Biofilms

Provisionally accepted
Xiaoyu Cao Xiaoyu Cao 1Ni Xiao Ni Xiao 2*Jingyi Huang Jingyi Huang 1*Li Li Li Li 2*Lian Zhong Lian Zhong 2*Jun Zhang Jun Zhang 2*Fengyun Wang Fengyun Wang 1*
  • 1 School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, Guangdong Province, China
  • 2 School of Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong Province, China

The final, formatted version of the article will be published soon.

    (1) Background: In the face of increasing antifungal resistance among Candida albicans biofilms, this study explores the efficacy of a combined treatment using Kangbainian lotion (KBN) and miconazole nitrate (MN) to address this challenge; (2) Methods: Using UPLC-Q-TOF/MS Analysis for Identification of Active Compounds in KBN Lotion; FICI for synergy evaluation, XTT and ROS assays for biofilm viability and oxidative stress, fluorescence and confocal laser scanning microscopy(CLSM) for structural and viability analysis, and real-time fluorescence for gene expression; (3) Conclusions: Our study indicates that the combined application of KBN and MN somewhat impacts the structural integrity of Candida albicans biofilms and affects the expression of several key genes involved in biofilm formation, including ALS1, ALS3, HWP1, HSP90, and CSH1. These preliminary findings suggest that there may be a synergistic effect between KBN and MN, potentially influencing not only the structural aspects of fungal biofilms but also involving the modulation of genetic pathways during their formation.

    Keywords: Candida albicans1, Biofilms2, KBN3, Miconazole Nitrate4, Synergistic5

    Received: 02 May 2024; Accepted: 31 Jul 2024.

    Copyright: © 2024 Cao, Xiao, Huang, Li, Zhong, Zhang and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Ni Xiao, School of Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou, 51006, Guangdong Province, China
    Jingyi Huang, School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, 510006, Guangdong Province, China
    Li Li, School of Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou, 51006, Guangdong Province, China
    Lian Zhong, School of Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou, 51006, Guangdong Province, China
    Jun Zhang, School of Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou, 51006, Guangdong Province, China
    Fengyun Wang, School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, 510006, Guangdong Province, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.