AUTHOR=Hochvaldova Lucie , Posselt Gernot , Wessler Silja , Kvítek Libor , Panáček Aleš 

TITLE=Implications of silver nanoparticles for H. pylori infection: modulation of CagA function and signaling

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=14

YEAR=2024

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2024.1419568

DOI=10.3389/fcimb.2024.1419568

ISSN=2235-2988

ABSTRACT=<sec><title>Background</title><p>Helicobacter pylori infection poses a significant health burden worldwide, and its virulence factor CagA plays a pivotal role in its pathogenesis.</p></sec><sec><title>Methods</title><p>In this study, the interaction between H. pylori-infected AGS cells and silver nanoparticles (AgNPs) was investigated, with a focus on the modulation of CagA-mediated responses, investigated by western blotting. Both, the dose-dependent efficacy against H. pylori (growth curves, CFU assay) and the impact of the nanoparticles on AGS cells (MTT assay) were elucidated.</p></sec><sec><title>Results</title><p>AGS cells infected with H. pylori displayed dramatic morphological changes, characterized by elongation and a migratory phenotype, attributed to CagA activity. Preincubation of H. pylori with AgNPs affected these morphological changes in a concentration-dependent manner, suggesting a correlation between AgNPs concentration and CagA function.</p></sec><sec><title>Conclusion</title><p>Our study highlights the nuanced interplay between host-pathogen interactions and the therapeutic potential of AgNPs in combating H. pylori infection and offers valuable insights into the multifaceted dynamics of CagA mediated responses.</p></sec>