AUTHOR=Shaik Shamshe , Lee Jin-Hyung , Kim Yong-Guy , Lee Jintae 

TITLE=Antifungal, anti-biofilm, and anti-hyphal properties of N-substituted phthalimide derivatives against Candida species

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=14

YEAR=2024

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2024.1414618

DOI=10.3389/fcimb.2024.1414618

ISSN=2235-2988

ABSTRACT=<p><italic>Candida</italic> species comprise a ubiquitous pathogenic fungal genus responsible for causing candidiasis. They are one of the primary causatives of several mucosal and systemic infections in humans and can survive in various environments. In this study, we investigated the antifungal, anti-biofilm, and anti-hyphal effects of six <italic>N</italic>-substituted phthalimides against three <italic>Candida</italic> species. Of the derivatives, <italic>N</italic>-butylphthalimide (NBP) was the most potent, with a minimum inhibitory concentration (MIC) of 100 µg/ml and which dose-dependently inhibited biofilm at sub-inhibitory concentrations (10–50 µg/ml) in both the fluconazole-resistant and fluconazole-sensitive <italic>Candida albicans</italic> and <italic>Candida parapsilosis</italic>. NBP also effectively inhibited biofilm formation in other pathogens including uropathogenic <italic>Escherichia coli</italic>, <italic>Staphylococcus epidermidis</italic>, <italic>Staphylococcus aureus</italic>, and <italic>Vibrio parahaemolyticus</italic>, along with the polymicrobial biofilms of <italic>S. epidermidis</italic> and <italic>C. albicans</italic>. NBP markedly inhibited the hyphal formation and cell aggregation of <italic>C. albicans</italic> and altered its colony morphology in a dose-dependent manner. Gene expression analysis showed that NBP significantly downregulated the expression of important hyphal- and biofilm-associated genes, i.e., <italic>ECE1</italic>, <italic>HWP1</italic>, and <italic>UME6</italic>, upon treatment. NBP also exhibited mild toxicity at concentrations ranging from 2 to 20 µg/ml in a nematode model. Therefore, this study suggests that NBP has anti-biofilm and antifungal potential against various <italic>Candida</italic> strains.</p>