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ORIGINAL RESEARCH article

Front. Cell. Infect. Microbiol.
Sec. Antibiotic Resistance and New Antimicrobial drugs
Volume 14 - 2024 | doi: 10.3389/fcimb.2024.1413024
This article is part of the Research Topic Evolution, Molecular Mechanisms and the Strategies to Combat Antimicrobial Resistance (AMR): A One Health Approach View all 21 articles

Whole-genome analysis of a ST45-SCCmec IVa (2B)-t116 methicillin-resistant Staphylococcus aureus strain isolated from the sputum of a 5-year-old child with pneumonia

Provisionally accepted
Lin Huang Lin Huang Rui Guo Rui Guo *Jingxian Lin Jingxian Lin Xiaowei Li Xiaowei Li *Zhicong Li Zhicong Li *Limei Zhang Limei Zhang *Wenting Li Wenting Li Cheng Zhang Cheng Zhang *Xiaosan Feng Xiaosan Feng *Xiaobin Li Xiaobin Li *
  • Zhuhai People’s Hospital (Zhuhai Clinical Medical College of Jinan University), Zhuhai, China

The final, formatted version of the article will be published soon.

    Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) sequence type (ST) 45 is a major global MRSA lineage with huge strain diversity and a high clinical impact. In Hainan and Guangzhou of China, the ST45-MRSA was mainly associated with t116.The MRSA strain SA2107 was isolated from the sputum of a 5-year-old child with pneumonia. The whole genome of SA2107 was sequence using Illumina (Novaseq 6000) and PacBio (Sequel IIe) sequencers, and the sequences were assembled using hybrid assembly. The carriage of antibiotic resistance genes, virulence genes, and mobile genetic elements were identified using bioinformatics tools. The comparative genomic analyses of MRSA strain SA2107 with other MRSA strains worldwide were performed.The genome size of ST45-SCCmec IVa (2B)-t116 MRSA strain SA2107 was ~2.9 Mb. genome. Mobile genetic elements analysis of SA2107 revealed two plasmids (30,064-bp pSA2107-1 and 8,033-bp pSA2107-2), three prophages, two integrative and conjugative elements (ICEs), and two insertion sequences (ISs, IS431 and IS1272). The SCCmec IVa (2B) carried by SA2107 contained the class B mec gene complex (IS431-mecA-ΔmecR1-IS1272) and type 2 ccr gene complex (ccrA2 and ccrB2). Besides mecA, another beta-lactam resistance gene blaZ was found to located on six copies of bla complex (blaZ, blaR1, and blaI) on the chromosome of SA2107. Three kinds of virulence factors were detected on the chromosome of SA2107, including genes encoding toxins, exoenzyme, and immune-modulating protein.Notably, the three prophages harbored by the chromosome of SA2107 all carried virulence genes.Thus far, only three complete genomes available for ST45-SCCmec IVa (2B)-t116 strain from United States, Germany, and Australia, respectively. The strain SA2107 was the first complete genome data (CP104559) from China for ST45-SCCmec IVa (2B)-t116 MRSA.

    Keywords: Methicillin-resistant Staphylococcus aureus (MRSA), ST45, SCCmec IVa, prophage, Virulence

    Received: 06 Apr 2024; Accepted: 26 Dec 2024.

    Copyright: © 2024 Huang, Guo, Lin, Li, Li, Zhang, Li, Zhang, Feng and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Rui Guo, Zhuhai People’s Hospital (Zhuhai Clinical Medical College of Jinan University), Zhuhai, China
    Xiaowei Li, Zhuhai People’s Hospital (Zhuhai Clinical Medical College of Jinan University), Zhuhai, China
    Zhicong Li, Zhuhai People’s Hospital (Zhuhai Clinical Medical College of Jinan University), Zhuhai, China
    Limei Zhang, Zhuhai People’s Hospital (Zhuhai Clinical Medical College of Jinan University), Zhuhai, China
    Cheng Zhang, Zhuhai People’s Hospital (Zhuhai Clinical Medical College of Jinan University), Zhuhai, China
    Xiaosan Feng, Zhuhai People’s Hospital (Zhuhai Clinical Medical College of Jinan University), Zhuhai, China
    Xiaobin Li, Zhuhai People’s Hospital (Zhuhai Clinical Medical College of Jinan University), Zhuhai, China

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