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ORIGINAL RESEARCH article

Front. Cell. Infect. Microbiol.
Sec. Bacteria and Host
Volume 14 - 2024 | doi: 10.3389/fcimb.2024.1401462

Probiotic Limosilactobacillus reuteri KUB-AC5 Decreases Urothelial Cell Invasion and Enhances Macrophage Killing of Uropathogenic Escherichia coli In Vitro Study

Provisionally accepted
Arishabhas Tantibhadrasapa Arishabhas Tantibhadrasapa 1Songbo Li Songbo Li 1,2Songphon Buddhasiri Songphon Buddhasiri 3,4Chutikarn Sukjoi Chutikarn Sukjoi 1Panupon Mongkolkarvin Panupon Mongkolkarvin 1Pattarapon Boonpan Pattarapon Boonpan 1Somsakul P. Wongpalee Somsakul P. Wongpalee 1Prasobsook Paenkaew Prasobsook Paenkaew 1Sawannee Sutheeworapong Sawannee Sutheeworapong 5Massalin Nakphaichit Massalin Nakphaichit 6,7Sunee Nitisinprasert Sunee Nitisinprasert 6,7Michael H. Hsieh Michael H. Hsieh 8Parameth Thiennimitr Parameth Thiennimitr 1,10,9*
  • 1 Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Chiang Mai, Thailand
  • 2 Youjiang Medical University for Nationalities, Baise, Guangx, China
  • 3 Research Center for Veterinary Biosciences and Veterinary Public Health, Chiang Mai University, Chiang Mai, Thailand
  • 4 Faculty of Veterinary Medicine, Chiang Mai University, Muang, Chiang Mai, Thailand
  • 5 Pilot Plant Development and Training Institute, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand
  • 6 Department of Biotechnology, Faculty of Agro Industry, Kasetsart University, Bangkok, Thailand
  • 7 Specialized Research Unit: Probiotics and Prebiotics for Health, Faculty of Agro-Industry, Kasetsart University, Bangkok, Thailand
  • 8 Department of Microbiology, Immunology, and Tropical Medicine, School of Medicine and Health Sciences, George Washington University, Washington DC, District of Columbia, United States
  • 9 Research Center of Microbial Diversity and Sustainable Utilization, Chiang Mai University, Chiang Mai, Thailand, Chiang Mai, Thailand
  • 10 Center of Multidisciplinary Technology for Advanced Medicine (CMUTEAM), Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand, Chiang Mai, Thailand

The final, formatted version of the article will be published soon.

    Bacterial urinary tract infections (UTI) are among the most common infectious diseases worldwide. The rise of multidrug-resistant (MDR) uropathogenic Escherichia coli (UPEC) UTI cases is a significant threat to healthcare systems. Several probiotic bacteria have been proposed as an alternative to combat MDR UTI. Lactic acid bacteria in the genus Limosilactobacillus are some of the most studied and used probiotics. However, strain-specific effects play a critical role in probiotic properties. L. reuteri KUB-AC5 (AC5), isolated from the chicken gut, confers antimicrobial and immunobiotic effects against some human pathogens. However, the antibacterial and immune modulatory effects of AC5 on UPEC have never been explored. Here, we investigated both the direct and indirect effects of AC5 against UPEC isolates (UTI89, CFT073, and clinical MDR UPEC AT31) in vitro. Using a spot-on lawn, agar-well diffusion, and competitive growth assays, we found that viable AC5 cells and cell-free components of this probiotic significantly reduced the UPEC growth of all strains tested. The human bladder epithelial cell line UM-UC-3 was used to assess the adhesion and pathogen-attachment inhibition properties of AC5 on UPEC. Our data showed that AC5 can attach to UM-UC-3 and decrease UPEC attachment in a dose-dependent manner.Pretreatment of UPEC-infected murine macrophage RAW264.7 cells with viable AC5 (multiplicity of infection, MOI = 1) for 24 hours enhanced macrophage-killing activity and increased proinflammatory (Nos2, Il6, and Tnfa) and anti-inflammatory (Il10) gene expression. These findings indicate the gut-derived AC5 probiotic could be a potential urogenital probiotic against MDR UTI.

    Keywords: Uropathogenic E. coli (UPEC), Urinary tract infection (UTI), probiotic, Limosilactobacillus reuteri, Immune Modulation, immunobiotic

    Received: 15 Mar 2024; Accepted: 01 Jul 2024.

    Copyright: © 2024 Tantibhadrasapa, Li, Buddhasiri, Sukjoi, Mongkolkarvin, Boonpan, Wongpalee, Paenkaew, Sutheeworapong, Nakphaichit, Nitisinprasert, Hsieh and Thiennimitr. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Parameth Thiennimitr, Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Chiang Mai, Thailand

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.