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ORIGINAL RESEARCH article

Front. Cell. Infect. Microbiol.
Sec. Biofilms
Volume 14 - 2024 | doi: 10.3389/fcimb.2024.1395577
This article is part of the Research Topic Fighting Microbial Biofilms: Novel Therapeutics and Antibiofilm Strategies View all 4 articles

PDIA Iminosugar Influence on Subcutaneous Staphylococcus aureus and Pseudomonas aeruginosa Infections in Mice

Provisionally accepted
Łucja Kozień Łucja Kozień 1Aleksandra Policht Aleksandra Policht 1*Piotr Heczko Piotr Heczko 1Zbigniew Arent Zbigniew Arent 2Urszula Bracha Urszula Bracha 2Laura Pardyak Laura Pardyak 2Agnieszka Pietsch-Fulbiszewska Agnieszka Pietsch-Fulbiszewska 2Estelle Gallienne Estelle Gallienne 3Piotr Piwowar Piotr Piwowar 4Krzysztof Okoń Krzysztof Okoń 1Anna Tomusiak-Plebanek Anna Tomusiak-Plebanek 5Magdalena Strus Magdalena Strus 1
  • 1 Jagiellonian University Medical College, Kraków, Lesser Poland, Poland
  • 2 University of Agriculture in Krakow, Krakow, Lesser Poland Voivodeship, Poland
  • 3 Université d'Orléans, Orléans, France
  • 4 Faculty of Electrical Engineering, Automatics, Computer Science and Biomedical Engineering, AGH University of Science and Technology, Kraków, Lesser Poland, Poland
  • 5 Department of Bacteriology, Ecology of Microbes and Parasitology, Faculty of Medicine, Jagiellonian University Medical College, Kraków, Poland

The final, formatted version of the article will be published soon.

    Biofilm-associated infections persist as a therapeutic challenge in contemporary medicine. The efficacy of antibiotic therapies is ineffective in numerous instances, necessitating a heightened focus on exploring novel anti-biofilm medical strategies. Among these, iminosaccharides, also recognized as iminosugars, emerge as a distinctive class of compounds displaying promising biofilm inhibition properties. This study employs an in vivo wound infection mouse model to evaluate the effectiveness of PDIA in treating biofilm-associated skin wound infections caused by Staphylococcus aureus and Pseudomonas aeruginosa. Dermic wounds in mice were infected with biofilm-forming strains, specifically S. aureus 48 and P. aeruginosa 5, which were isolated from patients with diabetic foot, and are well-known for their strong biofilm formation. The subsequent analysis included clinical, microbiological, and histopathological parameters.Furthermore, an exploration into the susceptibility of the infectious strains to hydrogen peroxide was conducted, acknowledging its potential presence during induced inflammation in mouse dermal wounds within an in vivo model. The findings revealed the efficacy of PDIA iminosugar against the S. aureus strain, evidenced by a reduction in bacterial numbers within the wound and the inflammatory focus. This study suggests that PDIA iminosugar emerges as an active and potentially effective antibiofilm agent, positioning it as a viable treatment option for staphylococcal infections.

    Keywords: antibiofilm drug, Wound Infection, Staphylococcus infection, Pseudomonas infection, PDIA iminosugar

    Received: 04 Mar 2024; Accepted: 11 Jul 2024.

    Copyright: © 2024 Kozień, Policht, Heczko, Arent, Bracha, Pardyak, Pietsch-Fulbiszewska, Gallienne, Piwowar, Okoń, Tomusiak-Plebanek and Strus. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Aleksandra Policht, Jagiellonian University Medical College, Kraków, 31-008, Lesser Poland, Poland

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.