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ORIGINAL RESEARCH article

Front. Cell. Infect. Microbiol.
Sec. Microbes and Innate Immunity
Volume 14 - 2024 | doi: 10.3389/fcimb.2024.1388035

Swine IFN cocktail can reduce mortality and lessen the tissue injury Caused by African swine fever virus infected piglets

Provisionally accepted
Jiang Yitong Jiang Yitong 1*Huang Ying Huang Ying 2Pang Zhongbao Pang Zhongbao 2*Zhai Wenzhu Zhai Wenzhu 2Chu Yuanyuan Chu Yuanyuan 2Jia hong Jia hong 2*Tao chunhang Tao chunhang 2*Zhu hongfei Zhu hongfei 2*
  • 1 Institute of Animal Sciences of CAAS, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China
  • 2 Institute of Animal Sciences of CAAS, Beijing, China

The final, formatted version of the article will be published soon.

    African swine fever (ASF), a highly virulent viral infection, poses a significant threat to the global pig industry. Currently, there are no commercially available vaccines against ASF. While the crucial role of Interferon (IFN) in combating viral infections is well established, its impact on clinical signs and mortality rates of ASF remains unclear. In this study, swine IFN-α2, IFN-γ, and IFN-λ3 were fused with the Fc segment of immunoglobulin G (IgG) and expressed in mammalian cells (293T), which improve the solubility and stability of these recombinant proteins. The specific activities of IFNα2-hFc, IFNγ-hFc and IFNλ3-hFc were determined to be 2.46x10 7 IU/mL, 4.54×10 9 IU/mL and 7.54×10 10 IU/mL, respectively. The IFNs-hFc significantly induced the expression of various IFNstimulated genes (ISGs), including IFIT5, Mx1, OASL, ISG12, STAT1, IRF1, PKR, CXCL10 and GBP1. Furthermore, treatment with the IFN cocktail (a mixture of IFNα2-hFc, IFNγ-hFc and IFNλ3-hFc) reduced the viral load, delayed death, and reduced tissue injury in piglets infected with ASFV. These results suggest that the combined use of the IFN cocktail may be effective in controlling and preventing the onset of ASF.

    Keywords: interferon, cocktail, African Swine Fever, Viral Load, Mortality

    Received: 19 Feb 2024; Accepted: 10 Jun 2024.

    Copyright: © 2024 Yitong, Ying, Zhongbao, Wenzhu, Yuanyuan, hong, chunhang and hongfei. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Jiang Yitong, Institute of Animal Sciences of CAAS, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China
    Pang Zhongbao, Institute of Animal Sciences of CAAS, Beijing, China
    Jia hong, Institute of Animal Sciences of CAAS, Beijing, China
    Tao chunhang, Institute of Animal Sciences of CAAS, Beijing, China
    Zhu hongfei, Institute of Animal Sciences of CAAS, Beijing, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.