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ORIGINAL RESEARCH article

Front. Cell. Infect. Microbiol.
Sec. Intestinal Microbiome
Volume 14 - 2024 | doi: 10.3389/fcimb.2024.1369192
This article is part of the Research Topic The Role of Gut Microbes and Their Metabolites in Immune-related Diseases-Volume II View all 11 articles

Gut Microbiome Characteristics of Women with Hypothyroidism During Early Pregnancy Detected by 16S rRNA Amplicon Sequencing and Shotgun Metagenomic

Provisionally accepted
Lin Hu Lin Hu Yajuan Xu Yajuan Xu *Jingjing Li Jingjing Li Miao Zhang Miao Zhang Zongzong Sun Zongzong Sun Yanjie Ban Yanjie Ban Xin Tian Xin Tian Dong Liu Dong Liu Lulu Hu Lulu Hu
  • Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China

The final, formatted version of the article will be published soon.

    Objective: This study aimed to explore the correlation between microbiota dysbiosis and hypothyroidism in early pregnancy by 16S rRNA amplicon sequencing combined with metagenomic sequencing.Methods: Sixty pregnant women (30 with hypothyroidism and 30 normal controls) were recruited for 16S rRNA amplicon sequencing, and 6 patients from each group were randomly selected for metagenomic sequencing to assess the gut microbiome profile.The 16S rRNA results showed that beta-diversity in the hypothyroidism group was decreased. The relative abundances of the Prevotella and Paraprevotella genera increased in the hypothyroidism group, and Blautia predominated in the controls. The metagenomics results revealed that Prevotella_stercorea_CAG_629, Prevotella_hominis, Prevotella_sp_AM34_19LB, etc. were enriched in the hypothyroidism group at the species level. Functional analysis revealed that the pyridoxal 5'-phosphate synthase pdxT subunit module was decreased, and the short-chain fatty acid (SCFA) transporter and phospholipase/carboxylesterase modules were strongly enriched in the hypothyroidism group. Hypothyroidism patients had increased C-reactive protein (CRP), interleukin-2 (IL-2), IL-4, IL-10, and tumor necrosis factor (TNF)-α levels. The pyridoxal 5'-phosphate synthase pdxT subunit, the SCFA transporter, and the phospholipase/carboxylesterase module were associated with different Prevotella species.Prevotella may affect the metabolism of glutamate, SCFA, and phospholipases, which could be involved in the development of hypothyroidism during pregnancy.

    Keywords: Hypothyroidism, Pregnancy, gut microbiome, 16S rRNA amplicon sequencing, Shotgun metagenomic sequencing

    Received: 11 Jan 2024; Accepted: 18 Jul 2024.

    Copyright: © 2024 Hu, Xu, Li, Zhang, Sun, Ban, Tian, Liu and Hu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Yajuan Xu, Third Affiliated Hospital of Zhengzhou University, Zhengzhou, China

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