AUTHOR=Osei Sekyere John , Mmatli Masego , Bosch Anel , Ntsoane Ramathetje Virginia , Naidoo Harishia , Doyisa Sinenhlanhla , Maningi Nontuthuko E. , Mbelle Nontombi Marylucy , Said Mohamed 

TITLE=Molecular epidemiology of multidrug-resistant Klebsiella pneumoniae, Enterobacter cloacae, and Escherichia coli outbreak among neonates in Tembisa hospital, South Africa

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=14

YEAR=2024

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2024.1328123

DOI=10.3389/fcimb.2024.1328123

ISSN=2235-2988

ABSTRACT=<sec><title>Background</title><p>An outbreak of multidrug-resistant <italic>Klebsiella pneumoniae, Escherichia coli, and Enterobacter cloacae</italic> infections in a neonatal ward within a tertiary hospital in South Africa resulted in the mortality of 10 patients within six months. In this work, the genomic epidemiology of and the molecular factors mediating this outbreak were investigated.</p></sec><sec><title>Methods</title><p>Bacterial cultures obtained from clinical samples collected from the infected neonates underwent phenotypic and molecular analyses to determine their species, sensitivity to antibiotics, production of carbapenemases, complete resistance genes profile, clonality, epidemiology, and evolutionary relationships. Mobile genetic elements flanking the resistance genes and facilitating their spread were also characterized.</p></sec><sec><title>Results</title><p>The outbreak was centered in two major wards and affected mainly neonates between September 2019 and March 2020. Most isolates (n = 27 isolates) were <italic>K. pneumoniae</italic> while both <italic>E. coli</italic> and <italic>E. cloacae</italic> had three isolates each. Notably, 33/34 isolates were multidrug resistant (MDR), with 30 being resistant to at least four drug classes. All the isolates were carbapenemase-positive, but four <italic>bla</italic><sub>OXA-48</sub> isolates were susceptible to carbapenems. <italic>Bla</italic><sub>NDM-1</sub> (n = 13) and <italic>bla</italic><sub>OXA-48/181</sub> (n = 15) were respectively found on <italic>IS</italic>91 and <italic>IS</italic>6-like <italic>IS</italic>26 composite transposons in the isolates alongside several other resistance genes. The repertoire of resistance and virulence genes, insertion sequences, and plasmid replicon types in the strains explains their virulence, resistance, and quick dissemination among the neonates.</p></sec><sec><title>Conclusions</title><p>The outbreak of fatal MDR infections in the neonatal wards were mediated by clonal (vertical) and horizontal (plasmid-mediated) spread of resistant and virulent strains (and genes) that have been also circulating locally and globally.</p></sec>