AUTHOR=Li Yini , Liu Xin , Chen Lingzhi , Shen Xiao , Wang Haihong , Guo Ruiyu , Li Xiang , Yu Zehui , Zhang Xiaoli , Zhou Yingshun , Fu Li 

TITLE=Comparative genomics analysis of Stenotrophomonas maltophilia strains from a community

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=13

YEAR=2023

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2023.1266295

DOI=10.3389/fcimb.2023.1266295

ISSN=2235-2988

ABSTRACT=<sec><title>Background</title><p><italic>Stenotrophomonas maltophilia</italic> is a multidrug-resistant (MDR) opportunistic pathogen with high resistance to most clinically used antimicrobials. The dissemination of MDR <italic>S. maltophilia</italic> and difficult treatment of its infection in clinical settings are global issues.</p></sec><sec><title>Methods</title><p>To provide more genetic information on <italic>S. maltophilia</italic> and find a better treatment strategy, we isolated five <italic>S. maltophilia</italic>, SMYN41–SMYN45, from a Chinese community that were subjected to antibiotic susceptibility testing, biofilm formation assay, and whole-genome sequencing. Whole-genome sequences were compared with other thirty-seven <italic>S. maltophilia</italic> sequences.</p></sec><sec><title>Results</title><p>The five <italic>S. maltophilia</italic> strains had similar antibiotic resistance profiles and were resistant to β-lactams, aminoglycosides, and macrolides. They showed similar antimicrobial resistance (AMR) genes, including various efflux pumps, β-lactamase resistance genes (<italic>blaL1/2</italic>), aminoglycoside resistance genes [<italic>aac(6’)</italic>, <italic>aph(3’/6)</italic>], and macrolide-resistant gene (<italic>MacB</italic>). Genome sequencing analysis revealed that SMYN41-SMYN45 belonged to sequence type 925 (ST925), ST926, ST926, ST31, and ST928, respectively, and three new STs were identified (ST925, ST926, and ST928).</p></sec><sec><title>Conclusion</title><p>This study provides genetic information by comparing genome sequences of several <italic>S. maltophilia</italic> isolates from a community of various origins, with the aim of optimizing empirical antibiotic medication and contributing to worldwide efforts to tackle antibiotic resistance.</p></sec>