AUTHOR=Logan Latania K. , Coy Laura Rojas , Pitstick Claire E. , Marshall Steven H. , Medernach Rachel L. , Domitrovic T. Nicholas , Konda Sreenivas , Qureshi Nadia K. , Hujer Andrea M. , Zheng Xiaotian , Rudin Susan D. , Weinstein Robert A. , Bonomo Robert A. 

TITLE=The role of the plasmid-mediated fluoroquinolone resistance genes as resistance mechanisms in pediatric infections due to Enterobacterales

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=13

YEAR=2023

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2023.1249505

DOI=10.3389/fcimb.2023.1249505

ISSN=2235-2988

ABSTRACT=<sec><title>Introduction</title><p>Fluoroquinolones (FQs) are not commonly prescribed in children, yet the increasing incidence of multidrug-resistant (MDR) Enterobacterales (Ent) infections in this population often reveals FQ resistance. We sought to define the role of FQ resistance in the epidemiology of MDR Ent in children, with an overall goal to devise treatment and prevention strategies.</p></sec><sec><title>Methods</title><p>A case–control study of children (0–18 years) at three Chicago hospitals was performed. Cases had infections by FQ-susceptible, β-lactamase-producing (<italic>bla</italic>) Ent harboring a non- or low-level expression of PMFQR genes (PMFQS Ent). Controls had FQR infections due to <italic>bla</italic> Ent with expressed PMFQR genes (PMFQR Ent). We sought <italic>bla</italic> genes by PCR or DNA (BD Max Check-Points assay<sup>®</sup>) and PMFQR genes by PCR. We performed rep-PCR, MLST, and <italic>E. coli</italic> phylogenetic grouping. Whole genome sequencing was additionally performed on PMFQS Ent positive isolates. Demographics, comorbidities, and device, antibiotic, and healthcare exposures were evaluated. Predictors of infection were assessed.</p></sec><sec><title>Results</title><p>Of 170 β-lactamase-producing Ent isolates, 85 (50%) were FQS; 23 (27%) had PMFQR genes (PMFQS cases). Eighty-five (50%) were FQR; 53 (62%) had PMFQR genes (PMFQR controls). The median age for children with PMFQS Ent and PMFQR Ent was 4.3 and 6.2 years, respectively (<italic>p</italic> = NS). Of 23 PMFQS Ent, 56% were <italic>Klebsiella</italic> spp., and of 53 PMFQR Ent, 76% were <italic>E. coli</italic>. The most common <italic>bla</italic> and PMFQR genes detected in PMFQS Ent were <italic>bla</italic><sub>SHV ESBL</sub> (44%) and <italic>oqxAB</italic> (57%), and the corresponding genes detected in PMFQR Ent were <italic>bla</italic><sub>CTX-M-1-group ESBL</sub> (79%) and <italic>aac(6’)-Ib-cr</italic> (83%). Whole genome sequencing of PMFQS Ent revealed the additional presence of <italic>mcr-9</italic>, a transferable polymyxin resistance gene, in 47% of isolates, along with multiple plasmids and mobile genetic elements propagating drug resistance. Multivariable regression analysis showed that children with PMFQS Ent infections were more likely to have hospital onset infection (OR 5.7, 95% CI 1.6–22) and isolates containing multiple <italic>bla</italic> genes (OR 3.8, 95% CI 1.1–14.5). The presence of invasive devices mediated the effects of healthcare setting in the final model. Differences in demographics, comorbidities, or antibiotic use were not found.</p></sec><sec><title>Conclusions</title><p>Paradoxically, PMFQS Ent infections were often hospital onset and PMFQR Ent infections were community onset. PMFQS Ent commonly co-harbored multiple <italic>bla</italic> and PMFQR genes, and additional silent, yet transferrable antibiotic resistance genes such as <italic>mcr-9</italic>, affecting therapeutic options and suggesting the need to address infection prevention strategies to control spread. Control of PMFQS Ent infections will require validating community and healthcare-based sources and risk factors associated with acquisition.</p></sec>