AUTHOR=Huang Yan-Ying , Li Qiu-Shi , Li Zhao-Dong , Sun Ai-Hua , Hu Sheng-Ping 

TITLE=Rapid diagnosis of Mycobacterium marinum infection using targeted nanopore sequencing: a case report

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=13

YEAR=2023

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2023.1238872

DOI=10.3389/fcimb.2023.1238872

ISSN=2235-2988

ABSTRACT=<p><italic>Mycobacterium marinum</italic> (<italic>M. marinum</italic>) is a non-tuberculous <italic>mycobacterium</italic> (NTM) that can cause infectious diseases in aquatic animals and humans. Culture-based pathogen detection is the gold standard for diagnosing NTM infection. However, this method is time-consuming and has low positivity rates for fastidious organisms. Oxford Nanopore MinION sequencing is an emerging third-generation sequencing technology that can sequence DNA or RNA directly in a culture-independent manner and offers rapid microbial identification. Further benefits include low cost, short turnaround time, long read lengths, and small equipment size. Nanopore sequencing plays a crucial role in assessing drug resistance, clinical identification of microbes, and monitoring infectious diseases. Some reports on <italic>Mycobacterium tuberculosis</italic> (MTB) using nanopore sequencing have been published, however, there are few reports on NTM, such as <italic>M. marinum</italic>. Here, we report the use of nanopore sequencing for the diagnosis of <italic>M. marinum</italic>.</p>