Dennis Nurjadi
Jan Tkadlec
Sébastien Boutin
François Vandenesch- 1Department of Infectious Diseases and Microbiology, University of Lübeck, Lübeck, Germany
- 2Department of Medical Microbiology, Charles University, 2nd Faculty of Medicine and Motol University Hospital, Prague, Czechia
- 3CIRI, Centre International de Recherche en Infectiologie, Université de Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, Centre National de la Recherche Scientifique (CNRS), UMR5308, École Normale Supérieure (ENS) de Lyon, Lyon, France
- 4Centre National de Référence des Staphylocoques, Institut des Agents Infectieux, Hospices Civils de Lyon, Lyon, France
Editorial on the Research Topic
“Omics”- revolution in elucidating the virulence and resistance in Staphylococcus aureus
Staphylococcus aureus is one of the most common causes of bacterial infections in humans, and the bacterial pathogen with the highest number of attributable deaths (Collaborators, 2022). The human nasal cavity serves as the natural reservoir for S. aureus. Although many healthy individuals are colonized with S. aureus, only some develop infections (Wertheim et al., 2005). The transition from a commensal to an opportunistic pathogen is not fully understood, but virulence factors likely play a significant role in initiating and facilitating infection processes (Howden et al., 2023). This Research Topic features articles showcasing the application of cutting-edge molecular biology methods (“Omics”) to elucidate the virulence and resistance of S. aureus.
The convergence of resistance and virulence is an intriguing phenomenon increasingly observed in many bacterial species (Li et al., 2021; Biggel et al., 2022). One of the archetypes of this convergence is the emergence of virulent community-associated methicillin-resistant S. aureus (MRSA), USA300 strains (Nimmo, 2012).To shed light on the events that shaped the evolution of this lineage, Bianco et al. investigated the evolution of the pandemic MRSA strain USA300 by analyzing and comparing genomic sequences of circulating USA300 strains and USA300 strains that predate the dominance of this expansive clone. They notably uncovered a pre-epidemic branching clade consisting of (already Panton-Valentine leukocidin (PVL)-positive) both methicillin-susceptible S. aureus (MSSA) and MRSA isolates circulating around the world that diverged from the USA300 lineage prior to the establishment of the South American and North American epidemics.
The treatment of infections caused by S. aureus can be challenging, as recurrences or chronicity may occur despite appropriate therapy (Tuchscherr et al., 2020). The study by Klein et al. found that even when belonging to the same clone, S. aureus isolated from different body sites and infection foci may exhibit differences in virulence and resistance phenotypes. This phenotypic plasticity and heterogeneity can be attributed to the integration of Sa3int bacteriophages into the β-hemolysin (hlb) gene, which results in the truncation of the hlb gene and the insertion of genes encoding staphylokinase (sak) and staphylococcal complement inhibitor (scn), leading to a highly plastic immune evasive phenotype.
Blocking bacterial virulence to promote pathogen killing and elimination by the immune system is an interesting alternative treatment approach (Ford et al., 2020). The study by Zhou et al. investigated the role of small RNA SprC using RNASeq for transcriptomics analysis on the metabolism and virulence of S. aureus N315. Over 2,497 identified transcripts, the SprC-mutant N315 S. aureus exhibited 23 downregulated differentially expressed genes, mainly related to metabolism and pathogenesis. Considering the emergence of drug resistance in S. aureus, such “pathoblockers” may be a promising alternative treatment strategy.
Traditionally, the clinical severity of S. aureus infections is associated with the presence or absence of certain genes coding some of the various S. aureus virulence factors (Howden et al., 2023). However, the impact of the expression levels of these virulence factors has been underexplored, largely due to the lack of high-throughput quantification methods for virulence proteins. In the study conducted by Pivard et al., the authors investigated the quantitative virulomes of 136 S. aureus isolates using a targeted proteomic approach. Their findings revealed that several virulence factors, including PVL, were associated with severity parameters in a dose-dependent manner, providing the proof of concept that “expression matters” in pathogen virulence and can be inferred from in vitro culture of the corresponding strain.
Nasal colonization with S. aureus is associated with an increased propensity to acquire infections (Bode et al., 2010). Therefore, understanding the mechanisms of persistent nasal colonization may help identify novel targets and strategies to decolonize high-risk patients. In their study, Salgado et al. used serial passaging of a murine colonization model and genome sequencing to demonstrate that changes were found in genes associated with the cell surface and metabolism, which might indicate niche adaptation in S. aureus to promote long-term colonization.
The articles presented in this Research Topic showcase the promising use of “OMICs” technologies in advancing research on S. aureus virulence and resistance. Specifically, the application of transcriptomics and proteomics adds a new functional and mechanistic dimension to elucidating the pathophysiology of S. aureus infections. By gaining a deeper understanding of the correlation between virulence factors and clinical outcomes, we may be able to improve diagnostic and therapeutic strategies for S. aureus infections.
Author contributions
All authors listed have made a substantial, direct, and intellectual contribution to the work and approved it for publication.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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References
Biggel, M., Moons, P., Nguyen, M. N., Goossens, H., Van Puyvelde, S. (2022). Convergence of virulence and antimicrobial resistance in increasingly prevalent escherichia coli ST131 papGII+ sublineages. Commun. Biol. 5, 752. doi: 10.1038/s42003-022-03660-x
Bode, L. G., Kluytmans, J. A., Wertheim, H. F., Bogaers, D., Vandenbroucke-Grauls, C. M., Roosendaal, R., et al. (2010). Preventing surgical-site infections in nasal carriers of staphylococcus aureus. New Engl. J. Med. 362, 9–17. doi: 10.1056/NEJMoa0808939
Collaborators, G.B.D.a.R (2022). Global mortality associated with 33 bacterial pathogens in 2019: a systematic analysis for the global burden of disease study 2019. Lancet 400, 2221–2248. doi: 10.1016/S0140-6736(22)02185-7
Ford, C. A., Hurford, I. M., Cassat, J. E. (2020). Antivirulence strategies for the treatment of staphylococcus aureus infections: a mini review. Front. Microbiol. 11, 632706. doi: 10.3389/fmicb.2020.632706
Howden, B. P., Giulieri, S. G., Wong Fok Lung, T., Baines, S. L., Sharkey, L. K., Lee, J. Y. H., et al. (2023). Staphylococcus aureus host interactions and adaptation. Nat. Rev. Microbiol. 1-16, 380–395. doi: 10.1038/s41579-023-00852-y
Li, Y., Hu, D., Ma, X., Li, D., Tian, D., Gong, Y., et al. (2021). Convergence of carbapenem resistance and hypervirulence leads to high mortality in patients with postoperative klebsiella pneumoniae meningitis. J. Glob Antimicrob. Resist. 27, 95–100. doi: 10.1016/j.jgar.2021.02.035
Nimmo, G. R. (2012). USA300 abroad: global spread of a virulent strain of community-associated methicillin-resistant staphylococcus aureus. Clin. Microbiol. Infect. 18, 725–734. doi: 10.1111/j.1469-0691.2012.03822.x
Tuchscherr, L., Loffler, B., Proctor, R. A. (2020). Persistence of staphylococcus aureus: multiple metabolic pathways impact the expression of virulence factors in small-colony variants (SCVs). Front. Microbiol. 11, 1028. doi: 10.3389/fmicb.2020.01028
Keywords: Staphylococcus aureus, omics, USA300, panton-valentine leukocidin, resistance and virulence
Citation: Nurjadi D, Tkadlec J, Boutin S and Vandenesch F (2023) Editorial: “Omics”- revolution in elucidating the virulence and resistance in Staphylococcus aureus. Front. Cell. Infect. Microbiol. 13:1209671. doi: 10.3389/fcimb.2023.1209671
Received: 21 April 2023; Accepted: 10 May 2023;
Published: 17 May 2023.
Edited and Reviewed by:
Jon Skare, Texas A&M Health Science Center, United StatesCopyright © 2023 Nurjadi, Tkadlec, Boutin and Vandenesch. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Dennis Nurjadi, ZGVubmlzLm51cmphZGlAdW5pLWx1ZWJlY2suZGU=