AUTHOR=Sun Ruizheng , Chen Hao , Yao Siqi , Yu Zheng , Lai Chen , Huang Jing 

TITLE=Ecological and dynamic analysis of gut microbiota in the early stage of azomethane-dextran sodium sulfate model in mice

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=13

YEAR=2023

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2023.1178714

DOI=10.3389/fcimb.2023.1178714

ISSN=2235-2988

ABSTRACT=<p>The success rate of azomethane-dextran sodium sulfate (AOM-DSS) model in mice has been a long-standing problem. Treatment of AOM and the first round DSS induces acute colitis and is of great significance for the success of AOM-DSS model. In this study, we focused on the role of gut microbiota in the early stage of AOM-DSS model. Few mice with obvious weight loss and high disease-activity score survived from double strike of AOM and the first round DSS. Different ecological dynamics of gut microbiota were observed in AOM-DSS treated mice. <italic>Pseudescherichia</italic>, <italic>Turicibacter</italic>, and <italic>Clostridium_XVIII</italic> were of significance in the model, uncontrolled proliferation of which accompanied with rapid deterioration and death of mice. <italic>Akkermansia</italic> and <italic>Ruthenibacterium</italic> were significantly enriched in the alive AOM-DSS treated mice. Decrease of <italic>Ligilactobacillus</italic>, <italic>Lactobacillus</italic>, and <italic>Limosilactobacillus</italic> were observed in AOM-DSS model, but significant drop of these genera could be lethal. <italic>Millionella</italic> was the only hub genus of gut microbiota network in dead mice, which indicated dysbiosis of the intestinal flora and fragility of microbial network. Our results will provide a better understanding for the role of gut microbiota in the early stage of AOM-DSS model and help improve the success rate of model construction.</p>