AUTHOR=Kiousi Despoina Eugenia , Efstathiou Christos , Tzampazlis Vasilis , Plessas Stavros , Panopoulou Maria , Koffa Maria , Galanis Alex 

TITLE=Genetic and phenotypic assessment of the antimicrobial activity of three potential probiotic lactobacilli against human enteropathogenic bacteria

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=13

YEAR=2023

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2023.1127256

DOI=10.3389/fcimb.2023.1127256

ISSN=2235-2988

ABSTRACT=<sec><title>Introduction</title><p>Lactobacilli are avid producers of antimicrobial compounds responsible for their adaptation and survival in microbe-rich matrices. The bactericidal or bacteriostatic ability of lactic acid bacteria (LAB) can be exploited for the identification of novel antimicrobial compounds to be incorporated in functional foodstuffs or pharmaceutical supplements. In this study, the antimicrobial and antibiofilm properties of <italic>Lactiplantibacillus pentosus</italic> L33, <italic>Lactiplantibacillus plantarum</italic> L125 and <italic>Lacticaseibacillus paracasei</italic> SP5, previously isolated form fermented products, were examined, against clinical isolates of <italic>Staphylococcus aureus</italic>, <italic>Salmonella enterica</italic> subsp. <italic>enterica</italic> serovar Enteritidis and <italic>Escherichia coli</italic>.</p></sec><sec><title>Methods</title><p>The ability of viable cells to inhibit pathogen colonization on HT-29 cell monolayers, as well as their co-aggregation capacity, were examined utilizing the competitive exclusion assay. The antimicrobial activity of cell-free culture supernatants (CFCS) was determined against planktonic cells and biofilms, using microbiological assays, confocal microscopy, and gene expression analysis of biofilm formation-related genes. Furthermore, <italic>in vitro</italic> analysis was supplemented with <italic>in silico</italic> prediction of bacteriocin clusters and of other loci involved in antimicrobial activity.</p></sec><sec><title>Results</title><p>The three lactobacilli were able to limit the viability of planktonic cells of <italic>S. aureus</italic> and <italic>E. coli</italic> in suspension. Greater inhibition of biofilm formation was recorded after co-incubation of <italic>S. enterica</italic> with the CFCS of <italic>Lc. paracasei</italic> SP5. Predictions based on sequence revealed the ability of strains to produce single or two-peptide Class II bacteriocins, presenting sequence and structural conservation with functional bacteriocins.</p></sec><sec><title>Discussion</title><p>The efficiency of the potentially probiotic bacteria to elicit antimicrobial effects presented a strain- and pathogen-specific pattern. Future studies, utilizing multi-omic approaches, will focus on the structural and functional characterization of molecules involved in the recorded phenotypes.</p></sec>