AUTHOR=Li Xinwei , Wang Yufei , Jiang Xuelian , Zeng Yuhao , Zhao Xinran , Washio Jumpei , Takahashi Nobuhiro , Zhang Linglin TITLE=Investigation of drug resistance of caries-related streptococci to antimicrobial peptide GH12 JOURNAL=Frontiers in Cellular and Infection Microbiology VOLUME=12 YEAR=2022 URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2022.991938 DOI=10.3389/fcimb.2022.991938 ISSN=2235-2988 ABSTRACT=
Dental caries is associated with caries-related streptococci and antimicrobial agents have been widely used for caries control, but troubled by antibiotic resistance. This study aimed to investigate the intrinsic and acquired resistance of caries-related streptococci to antimicrobial peptide GH12, which was proven promising for caries control, and preliminarily explore the phenotypic changes and whole genome of stable acquired resistant strains. In this study, susceptibility assays and resistance assays were performed, followed by stability assays of resistance, to evaluate the intrinsic resistance and the potential resistance of caries-related streptococci. Then, the phenotypic changes of the stable acquired resistant strain were explored. The whole genome of the resistant strain was sequenced and analyzed by second-generation and third-generation high-throughput sequencing technologies.