AUTHOR=Zhu Bo , Hu Juan , Li Xuelian , Li Xiaomin , Wang Lei , Fan Shihui , Jin Xin , Wang Kun , Zhao Weiguo , Zhu Wenjun , Chen Cheng , Wang Zilu , Lu Yingzhi TITLE=Rapid and specific detection of Enterococcus faecalis with a visualized isothermal amplification method JOURNAL=Frontiers in Cellular and Infection Microbiology VOLUME=12 YEAR=2022 URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2022.991849 DOI=10.3389/fcimb.2022.991849 ISSN=2235-2988 ABSTRACT=

Enterococcus faecalis is a serious problem for hospitals and can spread from patient to patient. Most of the current detection methods are associated with limitations associated with the need for trained personnel; they are also time-consuming. Thus, it is necessary to develop rapid and accurate detection methods to control the spread of E. faecalis. In this study, we developed a rapid and accurate detection method for E. faecalis using recombinase polymerase amplification (RPA) combined with a lateral flow strip (LFS). This method could be completed in approximately 35 min at 37°C. The limit of detection was 10 CFU/µL, irrespective of whether the templates were pure or complex. This method also showed good specificity and compatibility. In total, 278 clinical samples were tested using the RPA-LFS method; the detection accuracy was equal to that of the conventional qPCR method. This visualized isothermal amplification method could be useful for the future on-site detection of E. faecalis.