AUTHOR=Peng Yahui , Wei Jieling , Jia Xiaonan , Luan Feiyu , Man Mingyin , Ma Xiaohui , Luo Yinghao , Li Yue , Li Nana , Wang Qian , Wang Xibo , Zhou Yang , Ji Yuanyuan , Mu Wenjing , Wang Jun , Wang Chunying , Zhang Qianqian , Yu Kaijiang , Zhao Mingyan , Wang Changsong 

TITLE=Changes in the microbiota in different intestinal segments of mice with sepsis

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=12

YEAR=2023

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2022.954347

DOI=10.3389/fcimb.2022.954347

ISSN=2235-2988

ABSTRACT=<sec><title>Introduction</title><p>The small intestine, as the main digestion and absorption site of the gastrointestinal tract, is often overlooked in studies, and the overall microbiota does not reflect the makeup of the microbiota in different segments of the intestine. Therefore, we aimed to exclude the influence of routine ICU treatment measures on sepsis patients and observed changes in the diversity and abundance of gut microbiota in different intestinal segments of septic mice.</p></sec><sec><title>Methods</title><p>The mice were randomly divided into the CLP6h group and the sham group. The contents of the colon and small intestine of the experimental group and the control group were collected after 6 h.</p></sec><sec><title>Results</title><p>After CLP, the number and structure of the gut microbiota in the colon changed most obviously, among which Bacteroidetes had the most significant changes. <italic>Akkermansia</italic>, <italic>D.Firmicutes_bacterium_M10_2</italic>, <italic>Blautia</italic>, <italic>Bifidobacterium</italic>, <italic>Lactobacillus</italic>, <italic>Candidatus_Arthromitus</italic>, and <italic>Muribaculaceae</italic> were changed in the colon. <italic>Lactobacillus</italic>, <italic>Bifidobacterium</italic>, <italic>Akkermansia</italic>, <italic>Blautia</italic>, <italic>Candidatus_Arthromitus</italic>, and <italic>Lachnospiraceae_NK4A136_group</italic> were changed in the small intestine.</p></sec><sec><title>Discussion</title><p>Our experiment found that there were different numbers of unique and common gut microbiota in the small intestine and colon after sepsis, and the gut microbiota of the colon changed more drastically after sepsis than the small intestine. Thus, we should focus on protective gut microbiota and mucin-degrading microbes. We hope that these results will provide help for sepsis treatment in the future.</p></sec>