AUTHOR=Tian Chongmei , Xing Mengyu , Fu Liping , Zhao Yaping , Fan Xueyu , Wang Siwei 

TITLE=Emergence of uncommon KL38-OCL6-ST220 carbapenem-resistant Acinetobacter pittii strain, co-producing chromosomal NDM-1 and OXA-820 carbapenemases

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=12

YEAR=2022

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2022.943735

DOI=10.3389/fcimb.2022.943735

ISSN=2235-2988

ABSTRACT=<sec><title>Objective</title><p>To characterize one KL38-OCL6-ST220 carbapenem-resistant <italic>Acinetobacter pittii</italic> strain, co-producing chromosomal NDM-1 and OXA-820 carbapenemases.</p></sec><sec><title>Methods</title><p><italic>A. pittii</italic> TCM strain was isolated from a bloodstream infection (BSI). Antimicrobial susceptibility tests were conducted <italic>via</italic> disc diffusion and broth microdilution. Stability experiments of <italic>bla</italic><sub>NDM-1</sub> and <italic>bla</italic><sub>OXA-820</sub> carbapenemase genes were further performed. Whole-genome sequencing (WGS) was performed on the Illumina and Oxford Nanopore platforms. Multilocus sequence typing (MLST) was analyzed based on the Pasteur and Oxford schemes. Resistance genes, virulence factors, and insertion sequences (ISs) were identified with ABRicate based on ResFinder 4.0, virulence factor database (VFDB), and ISfinder. Capsular polysaccharide (KL), lipooligosaccharide outer core (OCL), and plasmid reconstruction were tested using <italic>Kaptive</italic> and PLACNETw. PHASTER was used to predict prophage regions. A comparative genomics analysis of all ST220 <italic>A. pittii</italic> strains from the public database was carried out. Point mutations, average nucleotide identity (ANI), DNA–DNA hybridization (DDH) distances, and pan-genome analysis were performed.</p></sec><sec><title>Results</title><p><italic>A. pittii</italic> TCM was ST220<sup>Pas</sup> and ST1818<sup>Oxf</sup> with KL38 and OCL6, respectively. It was resistant to imipenem, meropenem, and ciprofloxacin but still susceptible to amikacin, colistin, and tigecycline. WGS revealed that <italic>A. pittii</italic> TCM contained one circular chromosome and four plasmids. The Tn<italic>125</italic> composite transposon, including <italic>bla</italic><sub>NDM-1</sub>, was located in the chromosome with 3-bp target site duplications (TSDs). Many virulence factors and the <italic>bla</italic><sub>OXA-820</sub> carbapenemase gene were also identified. The stability assays revealed that <italic>bla</italic><sub>NDM-1</sub> and <italic>bla</italic><sub>OXA-820</sub> were stabilized by passage in an antibiotic-free medium. Moreover, 12 prophage regions were identified in the chromosome. Phylogenetic analysis showed that there are 11 ST220 <italic>A. pittii</italic> strains, and one collected from Anhui, China was closely related. All ST220 <italic>A. pittii</italic> strains presented high ANI and DDH values; they ranged from 99.85% to 100% for ANI and from 97.4% to 99.9% for DDH. Pan-genome analysis revealed 3,200 core genes, 0 soft core genes, 1,571 shell genes, and 933 cloud genes among the 11 ST220 <italic>A. pittii</italic> strains.</p></sec><sec><title>Conclusions</title><p>The coexistence of chromosomal NDM-1 and OXA-820 carbapenemases in <italic>A. pittii</italic> presents a huge challenge in healthcare settings. Increased surveillance of this species in hospital and community settings is urgently needed.</p></sec>