AUTHOR=Huang Yulan , Ma Xiaobo , Zeng Shihan , Fu Liang , Xu Heping , Li Xiaoyan 

TITLE=Emergence of a Salmonella Rissen ST469 clinical isolate carrying blaNDM-13 in China

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=12

YEAR=2022

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2022.936649

DOI=10.3389/fcimb.2022.936649

ISSN=2235-2988

ABSTRACT=<p>New Delhi metallo-β-lactamase-13 (NDM-13) is an NDM variant that was first identified in 2015 and has not been detected in <italic>Salmonella</italic> species prior to this study. Here we describe the first identification of a <italic>Salmonella</italic> Rissen strain SR33 carrying <italic>bla</italic><sub>NDM-13</sub>. The aim of this study was to molecularly characterize SR33’s antimicrobial resistance and virulence features as well as investigate the genetic environment of <italic>bla</italic><sub>NDM-13</sub>. The <italic>Salmonella</italic> Rissen SR33 strain was isolated from a patient with fever and diarrhea. SR33 belonged to ST469, and it was found to be multidrug-resistant (MDR) and to carry many virulence genes. Phylogenetic analysis showed that SR33 shared a close relationship with most of the Chinese <italic>S</italic>. Rissen ST469 strains. <italic>bla</italic><sub>NDM-13</sub> was located in a transmissible IncI1 plasmid pNDM13-SR33. Sequence analysis of <italic>bla</italic><sub>NDM-13</sub>-positive genomes downloaded from GenBank revealed that a genetic context (ΔIS<italic>Aba125</italic>-<italic>bla</italic><sub>NDM-13</sub>-<italic>ble</italic><sub>MBL</sub>-trpF) and a hybrid promoter (consisting of −35 sequences provided by IS<italic>Aba125</italic> and −10 sequences) were conserved. IS<italic>Aba125</italic> was truncated by IS<italic>1294</italic> in three plasmids carrying <italic>bla</italic><sub>NDM-13</sub>, including pNDM13-SR33. To our knowledge, this is the first report of <italic>bla</italic><sub>NDM-13</sub> carried by <italic>Salmonella</italic>. The emergence of <italic>bla</italic><sub>NDM-13</sub> in a clinical MDR <italic>S</italic>. Rissen ST469 strain highlights the critical need for monitoring and controlling the dissemination of <italic>bla</italic><sub>NDM-13</sub>. <italic>bla</italic><sub>NDM-13</sub> carried by a transmissible IncI1 plasmid may result in an increased risk of <italic>bla</italic><sub>NDM-13</sub> transmission. IS<italic>1294</italic> may be involved in the movement of <italic>bla</italic><sub>NDM-13</sub>.</p>