AUTHOR=Liu Wei , Jiang Pengcheng , Yang Keli , Song Qiqi , Yuan Fangyan , Liu Zewen , Gao Ting , Zhou Danna , Guo Rui , Li Chang , Sun Pei , Tian Yongxiang 

TITLE=Mycoplasma hyopneumoniae Infection Activates the NOD1 Signaling Pathway to Modulate Inflammation

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=12

YEAR=2022

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2022.927840

DOI=10.3389/fcimb.2022.927840

ISSN=2235-2988

ABSTRACT=<p><italic>Mycoplasma hyopneumoniae</italic> is a highly contagious pathogen causing porcine enzootic pneumonia, which elicits prolonged inflammatory response modulated by pattern recognition receptors (PRRs). Although significant advances have been achieved in understanding the Toll-Like receptors that recognize <italic>M. hyopneumoniae</italic>, the role of nucleotide-binding oligomerization domain 1 (NOD1) in <italic>M. hyopneumoniae</italic> infected cells remains poorly understood. This study revealed that <italic>M. hyopneumoniae</italic> activates the NOD1-RIP2 pathway and is co-localized with host NOD1 during infection. siRNA knockdown of NOD1 significantly impaired the TRIF and MYD88 pathway and blocked the activation of TNF-α. In contrast, NOD1 overexpression significantly suppressed <italic>M. hyopneumoniae</italic> proliferation. Furthermore, we for the first time investigated the interaction between <italic>M. hyopneumoniae</italic> mhp390 and NOD1 receptor, and the results suggested that mhp390 and NOD1 are possibly involved in the recognition of <italic>M. hyopneumoniae.</italic> These findings may improve our understanding of the interaction between PRRs and <italic>M. hyopneumoniae</italic> and the function of NOD1 in host defense against <italic>M. hyopneumoniae</italic> infection.</p>