AUTHOR=Loh Elizabeth H. , Nava Alessandra , Murray Kris A. , Olival Kevin J. , Guimarães Moisés , Shimabukuro Juliana , Zambrana-Torrelio Carlos , Fonseca Fernanda R. , de Oliveira Daniele Bruna Leal , Campos Angélica Cristine de Almeida , Durigon Edison L. , Ferreira Fernando , Struebig Matthew J. , Daszak Peter TITLE=Prevalence of bat viruses associated with land-use change in the Atlantic Forest, Brazil JOURNAL=Frontiers in Cellular and Infection Microbiology VOLUME=12 YEAR=2022 URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2022.921950 DOI=10.3389/fcimb.2022.921950 ISSN=2235-2988 ABSTRACT=Introduction

Bats are critical to maintaining healthy ecosystems and many species are threatened primarily due to global habitat loss. Bats are also important hosts of a range of viruses, several of which have had significant impacts on global public health. The emergence of these viruses has been associated with land-use change and decreased host species richness. Yet, few studies have assessed how bat communities and the viruses they host alter with land-use change, particularly in highly biodiverse sites.

Methods

In this study, we investigate the effects of deforestation on bat host species richness and diversity, and viral prevalence and richness across five forested sites and three nearby deforested sites in the interior Atlantic Forest of southern Brazil. Nested-PCR and qPCR were used to amplify and detect viral genetic sequence from six viral families (corona-, adeno-, herpes-, hanta-, paramyxo-, and astro-viridae) in 944 blood, saliva and rectal samples collected from 335 bats.

Results

We found that deforested sites had a less diverse bat community than forested sites, but higher viral prevalence and richness after controlling for confounding factors. Viral detection was more likely in juvenile males located in deforested sites. Interestingly, we also found a significant effect of host bat species on viral prevalence indicating that viral taxa were detected more frequently in some species than others. In particular, viruses from the Coronaviridae family were detected more frequently in generalist species compared to specialist species.

Discussion

Our findings suggest that deforestation may drive changes in the ecosystem which reduce bat host diversity while increasing the abundance of generalist species which host a wider range of viruses.