AUTHOR=Nie Fujiao , Wang Lihua , Huang Yingying , Yang Pishan , Gong Pizhang , Feng Qiang , Yang Chengzhe 

TITLE=Characteristics of Microbial Distribution in Different Oral Niches of Oral Squamous Cell Carcinoma

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=12

YEAR=2022

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2022.905653

DOI=10.3389/fcimb.2022.905653

ISSN=2235-2988

ABSTRACT=<p>Oral squamous cell carcinoma (OSCC), one of the most common malignant tumors of the head and neck, is closely associated with the presence of oral microbes. However, the microbiomes of different oral niches in OSCC patients and their association with OSCC have not been adequately characterized. In this study, 305 samples were collected from 65 OSCC patients, including tumor tissue, adjacent normal tissue (paracancerous tissue), cancer surface tissue, anatomically matched contralateral normal mucosa, saliva, and tongue coat. 16S ribosomal DNA (16S rDNA) sequencing was used to compare the microbial composition, distribution, and co-occurrence network of different oral niches. The association between the microbiome and the clinical features of OSCC was also characterized. The oral microbiome of OSCC patients showed a regular ecological distribution. Tumor and paracancerous tissues were more microbially diverse than other oral niches. Cancer surface, contralateral normal mucosa, saliva, and tongue coat showed similar microbial compositions, especially the contralateral normal mucosa and saliva. Periodontitis-associated bacteria of the genera <italic>Fusobacterium</italic>, <italic>Prevotella</italic>, <italic>Porphyromonas</italic>, <italic>Campylobacter</italic>, and <italic>Aggregatibacter</italic>, and anaerobic bacteria were enriched in tumor samples. The microbiome was highly correlated with tumor clinicopathological features, with several genera (<italic>Lautropia</italic>, <italic>Asteroleplasma</italic>, <italic>Parvimonas</italic>, <italic>Peptostreptococcus</italic>, <italic>Pyramidobacter</italic>, <italic>Roseburia</italic>, and <italic>Propionibacterium</italic>) demonstrating a relatively high diagnostic power for OSCC metastasis, potentially providing an indicator for the development of OSCC.</p>