AUTHOR=Ramos Luís Felipe Costa , Martins Michele , Murillo Jimmy Rodriguez , Domont Gilberto Barbosa , de Oliveira Danielle Maria Perpétua , Nogueira Fábio César Sousa , Maciel-de-Freitas Rafael , Junqueira Magno 

TITLE=Interspecies Isobaric Labeling-Based Quantitative Proteomics Reveals Protein Changes in the Ovary of Aedes aegypti Coinfected With ZIKV and Wolbachia

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=12

YEAR=2022

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2022.900608

DOI=10.3389/fcimb.2022.900608

ISSN=2235-2988

ABSTRACT=<p>Zika is a vector-borne disease caused by an arbovirus (ZIKV) and overwhelmingly transmitted by <italic>Ae. aegypti</italic>. This disease is linked to adverse fetal outcomes, mostly microcephaly in newborns, and other clinical aspects such as acute febrile illness and neurologic complications, for example, Guillain-Barré syndrome. One of the most promising strategies to mitigate arbovirus transmission involves releasing <italic>Ae. aegypti</italic> mosquitoes carrying the maternally inherited endosymbiont bacteria <italic>Wolbachia pipientis</italic>. The presence of <italic>Wolbachia</italic> is associated with a reduced susceptibility to arboviruses and a fitness cost in mosquito life-history traits such as fecundity and fertility. However, the mechanisms by which <italic>Wolbachia</italic> influences metabolic pathways leading to differences in egg production remains poorly known. To investigate the impact of coinfections on the reproductive tract of the mosquito, we applied an isobaric labeling-based quantitative proteomic strategy to investigate the influence of <italic>Wolbachia w</italic>Mel and ZIKV infection in <italic>Ae. aegypti</italic> ovaries. To the best of our knowledge, this is the most complete proteome of <italic>Ae. aegypti</italic> ovaries reported so far, with a total of 3913 proteins identified, were also able to quantify 1044 <italic>Wolbachia</italic> proteins in complex sample tissue of <italic>Ae. aegypti</italic> ovary. Furthermore, from a total of 480 mosquito proteins modulated in our study, we discuss proteins and pathways altered in <italic>Ae. aegypti</italic> during ZIKV infections, <italic>Wolbachia</italic> infections, coinfection <italic>Wolbachia</italic>/ZIKV, and compared with no infection, focusing on immune and reproductive aspects of <italic>Ae. aegypti</italic>. The modified aspects mainly were related to the immune priming enhancement by <italic>Wolbachia</italic> presence and the modulation of the Juvenile Hormone pathway caused by both microorganism’s infection.</p>