AUTHOR=Ramalingam Vaikundamoorthy , Rajaram Rajendran , Archunan Govindaraju , Padmanabhan Parasuraman , Gulyás Balázs 

TITLE=Structural Characterization, Antimicrobial, Antibiofilm, Antioxidant, Anticancer and Acute Toxicity Properties of N-(2-hydroxyphenyl)-2-phenazinamine From Nocardiopsis exhalans (KP149558)

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=12

YEAR=2022

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2022.794338

DOI=10.3389/fcimb.2022.794338

ISSN=2235-2988

ABSTRACT=<p>The present study aimed to isolate and identify potential drugs from marine actinomycete <italic>Nocardiopsis exhalans</italic> and screen them for biomedical applications. The cell-free culture of <italic>N.</italic> exhalans was extracted with ethyl acetate and the solvent extract showed six fractions in thin-layer chromatography. The fractions were subjected to column chromatography for purification and evaluated for activity against human clinical pathogens. Fraction 4 showed significant activity and was identified as N-(2-hydroxyphenyl)-2-phenazinamine (NHP) using spectral analyses. Further, NHP showed excellent biofilm inhibitory activity against human clinical pathogens <italic>Escherichia coli</italic>, <italic>Pseudomonas aeruginosa</italic>, and <italic>Staphylococcus aureus</italic>. The <italic>in vitro</italic> antioxidant activity confirmed that NHP is scavenging the oxidative stress-enhancing molecules. The anti-proliferative activity of NHP against human breast cancer cells showed significant activity at 300 µg/ml and less cytotoxic activity against normal cells. Additionally, the toxicity assessment against zebrafish revealed that NHP does not cause any toxicity in the important organs. The results highlight <italic>N. exhalans</italic> as a promising candidate for the development of antibiotics with potential therapeutic applications.</p>