AUTHOR=Hidi László , Kovács Gergely Imre , Szabó Dóra , Makra Nóra , Pénzes Kinga , Juhász János , Sótonyi Péter , Ostorházi Eszter 

TITLE=Human blood vessel microbiota in healthy adults based on common femoral arteries of brain-dead multi-organ donors

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=12

YEAR=2022

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2022.1056319

DOI=10.3389/fcimb.2022.1056319

ISSN=2235-2988

ABSTRACT=<p>Discovery of human microbiota is fundamentally changing our perceptions of certain diseases and their treatments. However little is known about the human blood vessel microbiota, it may have important effects on vascular pathological lesions and vascular homograft failure. In our prospective survey study fourteen femoral arteries, harvested from donors in multi-organ donations, were examined using the V3-V4 region 16S rRNA sequencing method. The most abundant phyla in the human vascular microbiota were <italic>Proteobacteria</italic>, <italic>Firmicutes</italic> and <italic>Actinobacteria</italic>. At the genus level, the most abundant taxa were <italic>Staphylococcus</italic>, <italic>Corynebacterium</italic>, <italic>Pseudomonas</italic>, <italic>Bacillus</italic>, <italic>Acinetobacter</italic> and <italic>Propionibacterium</italic>. Of the bacterial taxa that have an indirect effect on the development of atherosclerosis, we found <italic>Porphyromonas gingivalis</italic>, <italic>Prevotella nigrescens</italic> and <italic>Enterobacteriaceae</italic> spp. with different abundances in our samples. Of the bacteria that are more common in the intestinal flora of healthy than of atherosclerosis patients, <italic>Roseburia</italic> and <italic>Ruminococcus</italic> occurred in the majority of samples. The human arterial wall has a unique microbiota that is significantly different in composition from that of other areas of the body. Our present study provides a basis for ensuing research that investigates the direct role of the microbiota in vascular wall abnormalities and the success of vascular allograft transplantations. </p>