AUTHOR=Zhou Mingming , Shi Qiucheng , Zhang Xiucai , Mei Lingling , Ye Yihua , Fang Chao , Shang Shiqiang 

TITLE=Salmonella enterica subsp. diarizonae Harboring ST233, ST1263, and ST1845 in Children

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=11

YEAR=2021

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2021.727811

DOI=10.3389/fcimb.2021.727811

ISSN=2235-2988

ABSTRACT=<sec><title>Objective</title><p>This study aims to analyze the molecular epidemiology, resistance, and pathogenicity of <italic>Salmonella enterica</italic> subsp. <italic>diarizonae</italic> isolated from children.</p></sec><sec><title>Methods</title><p>Whole genome sequencing was carried out, and molecular serotypes, sequence types, resistance genes, and virulence genes of <italic>S. enterica</italic> subsp. <italic>diarizonae</italic> isolates were analyzed. Antimicrobial susceptibility test was determined by commercialized microdilution method.</p></sec><sec><title>Results</title><p>A total of three isolates of <italic>S. enterica</italic> subsp. <italic>diarizonae</italic> were isolated during 2015 to 2020. The molecular serotypes of the three strains were 61:c:z35, 61:l,v:1,5,7:[z57], and 65:k:z, respectively, and the sequence types were ST1845, ST233, and ST1263. All the three isolates were susceptible to ceftriaxone, ceftazidime, cefepime, amoxycillin/clavulanic acid, piperacillin/tazobactam, ertapenem, imipenem, levofloxacin, and trimethoprim/sulfamethoxazole. No other resistant gene was detected except <italic>aac(6’)-Iaa</italic>. There were no resistant plasmids detected in all the three isolates. A total of 76 genes were present in all isolates, containing 49 genes of Type III Secretion System (T3SS) mediated by SPI-1and SPI-2, 13 genes of adherence (type 1 fimbriae, Agf, and MisL-related genes), 11 genes of iron uptake (Yersiniabactin), two genes of magnesium uptake, and one gene of typhoid toxin(<italic>cdtB</italic>).</p></sec><sec><title>Conclusion</title><p>The serotypes and sequence types of <italic>S. enterica</italic> subsp. <italic>diarizonae</italic> isolates were rarely reported in children; all the <italic>S. enterica</italic> subsp. <italic>diarizonae</italic> isolates were susceptible to detected antibiotics; T3SS, adherence, iron uptake, magnesium uptake, and typhoid toxin were responsible for pathogenicity of the <italic>S. enterica</italic> subsp. <italic>diarizonae</italic> isolates in children.</p></sec>