AUTHOR=Pallos Debora , Sousa Vanessa , Feres Magda , Retamal-Valdes Belen , Chen Tsute , Curtis Mike , Boaventura Richardson Mondego , Tanaka Marcia Hiromi , Salomão Gustavo Vargas da Silva , Zanella Louise , Tozetto-Mendoza Tania Regina , Schwab Gabriela , Franco Lucas Augusto Moyses , Sabino Ester Cerdeira , Braz-Silva Paulo Henrique , Shibli Jamil Awad 

TITLE=Salivary Microbial Dysbiosis Is Associated With Peri-Implantitis: A Case-Control Study in a Brazilian Population

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=11

YEAR=2022

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2021.696432

DOI=10.3389/fcimb.2021.696432

ISSN=2235-2988

ABSTRACT=<sec><title>Background and Objectives</title><p>The aim of this study was to examine the salivary microbiome in healthy peri-implant sites and those with peri-implantitis.</p></sec><sec><title>Methods</title><p>Saliva samples were collected from 21 participants with healthy peri-implant sites and 21 participants with peri-implantitis. The V4 hypervariable region of the 16S rRNA gene was sequenced using the Ion Torrent PGM System (Ion 318™ Chip v2 400). The NGS analysis and composition of the salivary microbiome were determined by taxonomy assignment. Downstream bioinformatic analyses were performed in QIIME (v 1.9.1).</p></sec><sec><title>Results</title><p>Clinical differences according to peri-implant condition status were found. Alpha diversity metrics revealed that the bacterial communities of participants with healthy peri-implant sites tended to have a richer microbial composition than individuals with peri-implantitis. In terms of beta diversity, bleeding on probing (BoP) may influence the microbial diversity. However, no clear partitioning was noted between the salivary microbiome of volunteers with healthy peri-implant sites or volunteers with peri-implantitis. The highest relative abundance of <italic>Stenotrophomonas</italic>, <italic>Enterococcus</italic> and <italic>Leuconostoc</italic> genus, and <italic>Faecalibacterium prausnitzii</italic>, <italic>Haemophilus parainfluenzae</italic>, <italic>Prevotella copri</italic>, <italic>Bacteroides vulgatus</italic>, and <italic>Bacteroides stercoris bacterial species</italic> was found in participants with peri-implantitis when compared with those with healthy peri-implant sites.</p></sec><sec><title>Conclusion</title><p>Differences in salivary microbiome composition were observed between patients with healthy peri-implant sites and those with peri-implantitis. BoP could affect the diversity (beta diversity) of the salivary microbiome.</p></sec>