AUTHOR=Gazzaniga Francesca S. , Camacho Diogo M. , Wu Meng , Silva Palazzo Matheus F. , Dinis Alexandre L. M. , Grafton Francis N. , Cartwright Mark J. , Super Michael , Kasper Dennis L. , Ingber Donald E. TITLE=Harnessing Colon Chip Technology to Identify Commensal Bacteria That Promote Host Tolerance to Infection JOURNAL=Frontiers in Cellular and Infection Microbiology VOLUME=11 YEAR=2021 URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2021.638014 DOI=10.3389/fcimb.2021.638014 ISSN=2235-2988 ABSTRACT=
Commensal bacteria within the gut microbiome contribute to development of host tolerance to infection, however, identifying specific microbes responsible for this response is difficult. Here we describe methods for developing microfluidic organ-on-a-chip models of small and large intestine lined with epithelial cells isolated from duodenal, jejunal, ileal, or colon organoids derived from wild type or transgenic mice. To focus on host-microbiome interactions, we carried out studies with the mouse Colon Chip and demonstrated that it can support co-culture with living gut microbiome and enable assessment of effects on epithelial adhesion, tight junctions, barrier function, mucus production, and cytokine release. Moreover, infection of the Colon Chips with the pathogenic bacterium,