AUTHOR=Wang Ping , Li Ren-Qing , Wang Lei , Yang Wen-Tao , Zou Qing-Hua , Xiao Di 

TITLE=Proteomic Analyses of Acinetobacter baumannii Clinical Isolates to Identify Drug Resistant Mechanism

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=11

YEAR=2021

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2021.625430

DOI=10.3389/fcimb.2021.625430

ISSN=2235-2988

ABSTRACT=<p><italic>Acinetobacter baumannii</italic> is one of the main causes of nosocomial infections. Increasing numbers of multidrug-resistant <italic>Acinetobacter baumannii</italic> cases have been reported in recent years, but its antibiotic resistance mechanism remains unclear. We studied 9 multidrug-resistant (MDR) and 10 drug-susceptible <italic>Acinetobacter baumannii</italic> clinical isolates using Label free, TMT labeling approach and glycoproteomics analysis to identify proteins related to drug resistance. Our results showed that 164 proteins exhibited different expressions between MDR and drug-susceptible isolates. These differential proteins can be classified into six groups: a. proteins related to antibiotic resistance, b. membrane proteins, membrane transporters and proteins related to membrane formation, c. Stress response-related proteins, d. proteins related to gene expression and protein translation, e. metabolism-related proteins, f. proteins with unknown function or other functions containing biofilm formation and virulence. In addition, we verified seven proteins at the transcription level in eight clinical isolates by using quantitative RT-PCR. Results showed that four of the selected proteins have positive correlations with the protein level. This study provided an insight into the mechanism of antibiotic resistance of multidrug-resistant <italic>Acinetobacter baumannii</italic>.</p>