AUTHOR=Zhang Xinmei , Li Chao , Cao Weijun , Zhang Zhenyu 

TITLE=Alterations of Gastric Microbiota in Gastric Cancer and Precancerous Stages

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=11

YEAR=2021

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2021.559148

DOI=10.3389/fcimb.2021.559148

ISSN=2235-2988

ABSTRACT=<sec><title>Objective</title><p>Microbial infections have been shown to contribute to gastric carcinogenesis, the knowledge of gastric microbiota alteration in this process may provide help in early diagnosis of gastric cancer. The aim of this study was to characterize the microbial changes and identify taxonomic biomarkers across stages of gastric carcinogenesis.</p></sec><sec><title>Methods</title><p>The gastric microbiota was investigated by 16S rRNA gene analysis in gastric mucosal specimens from 47 patients including superficial gastritis (SG), atrophic gastritis (AG), gastric intraepithelial neoplasia (GIN), and gastric cancer (GC). Differences in microbial composition across the disease stages, especially in GIN and GC were assessed using linear discriminant analysis effect size.</p></sec><sec><title>Results</title><p>There was no gradual changing trend in the richness or diversity of the gastric microbiota across stages of gastric carcinogenesis. The relative abundance of dominant taxa at phylum and genus levels didn’t show a gradual shift pattern, and the only four taxa that continuously enriched from SG to GC were <italic>Slackia</italic>, <italic>Selenomonas</italic>, <italic>Bergeyella</italic>, and <italic>Capnocytophaga</italic>, all of which were oral bacteria. The most representative taxa which were enriched in GC patients were oral bacteria including <italic>Parvimonas</italic>, <italic>Eikenella</italic> and <italic>Prevotella-2</italic>, and environmental bacteria including <italic>Kroppenstedtia</italic>, <italic>Lentibacillus</italic>, and <italic>Oceanobacillus</italic>. The gastric microbiota in GIN patients were characterized by enrichment of intestinal commensals including <italic>Romboutsia</italic>, <italic>Fusicatenibacter</italic>, <italic>Prevotellaceae-Ga6A1-group</italic>, and <italic>Intestinimonas</italic>. Gastric cardia cancer and non-cardia cancer patients had significantly different microbiota profiles characterized by a higher abundance of <italic>Helicobacter</italic> in the cardia cancer patients.</p></sec><sec><title>Conclusions</title><p>Our results provide insights on potential taxonomic biomarkers for gastric cancer and precancerous stages, and suggest that gastric microbiota might play different roles in the carcinogenesis of cardia cancer and non-cardia cancer.</p></sec>