AUTHOR=Slonova Darya , Posvyatenko Alexandra , Kibardin Alexey , Sysolyatina Elena , Lyssuk Elena , Ermolaeva Svetlana , Obydennyi Sergei , Gnuchev Nikolay , Georgiev Georgii , Severinov Konstantin , Larin Sergey 

TITLE=Human Short Peptidoglycan Recognition Protein PGLYRP1/Tag-7/PGRP-S Inhibits Listeria monocytogenes Intracellular Survival in Macrophages

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=10

YEAR=2020

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2020.582803

DOI=10.3389/fcimb.2020.582803

ISSN=2235-2988

ABSTRACT=<p>PGLYRP1/Tag-7/PGRP-S is one of mammalian peptidoglycan recognition proteins (PGRPs). Here, we demonstrate that human recombinant PGLYRP1/Tag-7/PGRP-S potentiates the response of murine macrophage-like ANA-1 cells and human macrophages to facultative intracellular pathogen <italic>Listeria monocytogenes</italic>. PGLYRP1/Tag-7/PGRP-S binds to the surface of <italic>L. monocytogenes</italic> and other bacterial cells but has no effect on their growth in culture. While PGLYRP1/Tag-7/PGRP-S treatment modestly enhanced phagocytosis of bacteria by ANA-1 cells, the intracellular survival of PGLYRP1/Tag-7/PGRP-S treated <italic>L. monocytogenes</italic> was strongly inhibited 2 h after internalization. PGLYRP1/Tag-7/PGRP-S treatment of bacteria boosted oxidative burst induction and increased the level of proinflammatory cytokine IL-6 produced by ANA-1, however, these effects happened too late to be responsible for decreased intracellular survival of bacteria. Our results thus suggest that PGLYRP1/Tag-7/PGRP-S acts as a molecular sensor for detection of <italic>L. monocytogenes</italic> infection of mammalian cells that leads to increased killing through a mechanism(s) that remains to be defined.</p>