Luz H. Patino
Marina Muñoz
Lissa Cruz-Saavedra1
Carlos Muskus
Juan David Ramírez- 1Grupo de Investigaciones Microbiológicas-UR (GIMUR), Departamento de Biología, Facultad de Ciencias Naturales, Universidad del Rosario, Bogotá, Colombia
- 2Programa de Estudio y Control de Enfermedades Tropicales (PECET), Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia
Leishmania (Viannia) braziliensis is an important Leishmania species circulating in several Central and South American countries. Among Leishmania species circulating in Brazil, Argentina and Colombia, L. braziliensis has the highest genomic variability. However, genomic variability at the whole genome level has been only studied in Brazilian and Peruvian isolates; to date, no Colombian isolates have been studied. Considering that in Colombia, L. braziliensis is a species with great clinical and therapeutic relevance, as well as the role of genetic variability in the epidemiology of leishmaniasis, we analyzed and evaluated intraspecific genomic variability of L. braziliensis from Colombian and Bolivian isolates and compared them with Brazilian isolates. Twenty-one genomes were analyzed, six from Colombian patients, one from a Bolivian patient, and 14 Brazilian isolates downloaded from public databases. The results obtained of Phylogenomic analysis showed the existence of four well-supported clades, which evidenced intraspecific variability. The whole-genome analysis revealed structural variations in the somy, mainly in the Brazilian genomes (clade 1 and clade 3), low copy number variations, and a moderate number of single-nucleotide polymorphisms (SNPs) in all genomes analyzed. Interestingly, the genomes belonging to clades 2 and 3 from Colombia and Brazil, respectively, were characterized by low heterozygosity (~90% of SNP loci were homozygous) and regions suggestive of loss of heterozygosity (LOH). Additionally, we observed the drastic whole genome loss of heterozygosity and possible hybridization events in one genome belonging to clade 4. Unique/shared SNPs between and within the four clades were identified, revealing the importance of some of them in biological processes of L. braziliensis. Our analyses demonstrate high genomic variability of L. braziliensis in different regions of South America, mainly in Colombia and suggest that this species exhibits striking genomic diversity and a capacity of genomic hybridization; additionally, this is the first study to report whole-genome sequences of Colombian L. braziliensis isolates.
Introduction
Leishmania (Viannia) braziliensis is the most important Leishmania species associated with cases of cutaneous leishmaniasis and muco-cutaneous leishmaniasis in several Central and South American countries (Ramirez et al., 2016; Patino et al., 2017). This species is characterized by its ability to cause distinct forms of tegumentary leishmaniasis in humans (Jirmanus et al., 2012; Meireles et al., 2017) and animals (De Oliveira et al., 2013; Brilhante et al., 2019), and also for its variable infectivity, virulence, and response to anti-leishmanial therapy (Rego et al., 2018; Patino et al., 2019a). In addition, it is considered a zoonotic parasite circulating in a wide range of mammalian and vector host species (Kuhls et al., 2013; Roque and Jansen, 2014), characteristics that can contribute to the generation and maintenance of genetic diversity within the species (Cupolillo et al., 2003; Bruna et al., 2019).
Several nuclear and mitochondrial DNA markers as well as different molecular techniques have been used with the purpose to evaluate inter- and intra-specific genetic variability of New/Old world Leishmania species, such as L. major, L.infantum, L. donovani, L. tropica, L. braziliensis, L. peruviana, L. guyanensis, and L. panamensis (Cupolillo et al., 2003; Nolder et al., 2007; Kuhls et al., 2013; Restrepo et al., 2013; Marco et al., 2015; Restrepo et al., 2015; Fotouhi-Ardakani et al., 2016; Cysne-Finkelstein et al., 2018; Quaresma et al., 2018; Banu et al., 2019). This has suggested high intra-specific variability, especially for L. braziliensis from Brazil (Marlow et al., 2014), Argentina (Marco et al., 2015) and Colombia (Herrera et al., 2017). This variability is probably related to sand fly vector(s) and/or animal reservoir(s) involved in transmission cycles (Cupolillo et al., 2003) and with different clinical manifestations (Quaresma et al., 2018; Rego et al., 2018). However, few studies have examined this variability in whole genomes of New-World Leishmania species, such as L. braziliensis (Valdivia et al., 2015; Urrea et al., 2018; Bruna et al., 2019; Restrepo et al., 2019), because most of the research has focused on revealing the genome diversity of Old-World Leishmania species (Downing et al., 2011; Imamura et al., 2016; Ghouila et al., 2017; Franssen et al., 2020).
Two recent studies have used whole genome sequencing to reveal the genetic variability of L. braziliensis. One of them, published by Bruna et al. (2019) highlighted the tremendous genetic variability (~95,000–~131,000 SNPs) in 10 clinical isolates from forested and urbanized environments of Brazil and the existence of three distinct phylogenetic groups including one isolate from a forested environment that was characterized by moderate aneuploidy and reduced heterozygosity. The other study by Broeck et al. (2020) investigated the nuclear and mitochondrial genomes of L. braziliensis isolates from Peru and demonstrated genetic diversification and subsequent hybridization, this study highlights the origin of Andean/Amazonian Leishmania species (L. peruviana and L. braziliensis) and describes a possible meiotic recombination event between them, with uniparental inheritance of maxicircles but biparental inheritance of minicircles, which may be crucial for survival of the parasite in the wild. Considering the close relatedness between L. braziliensis and L. peruviana, some authors have conducted genomic comparative analysis, which have identified a great number of interspecific SNP/indel differences between them as well as the presence of different gene and chromosome copy number variations supporting the classification of both organisms as closely related but distinct species (Valdivia et al., 2015; Broeck et al., 2020).
Despite these findings, knowledge about the genomic variability of L. braziliensis in other South American regions, such as Colombia, is limited. Different studies describe a high frequency of this species in Colombia; this being more frequent in rural (Perez-Franco et al., 2016; Patino et al., 2017) compared with urban populations (Ramirez et al., 2016; Ovalle-Bracho et al., 2019). In addition, Colombia has a large number of sand fly vectors and animal reservoirs, which are involved in the transmission cycle of the parasite (Valderrama-Ardila et al., 2010; Ferro et al., 2015). Considering the public health importance of leishmaniasis caused by L. braziliensis in Colombia and the role of genetic variability of the parasite in the epidemiology of the disease, the objective of this study was to perform a comprehensive analysis of whole genome sequencing (DNA-seq) to evaluate phylogenomic relationships among six Colombian clinical isolates and one Bolivian clinical isolate. We also determined phylogenomic relationships and genomic plasticity among these isolates and 14 publicly available L. braziliensis genomes from Brazil in terms of genome plasticity (chromosomes/genes) and single point mutations.
Methods
Ethics Statement
All procedures were approved by the Ethics committee of the Universidad de Antioquia (number VRI3445/2010) in accordance with resolution number 36836. The patients included into the study read and signed the informed consent.
Population of Study
Twenty-one whole genomes were analyzed in this study, including six from Colombian clinical isolates and one Bolivian clinical isolate from patients who were attending in the ‘Programa de Estudio y Control de Enfermedades Tropicales (PECET), Medellín-Colombia’. Also, 14 L. braziliensis genomic sequences reads, from Brazilian isolates publicly available from the DDBJ/ENA/GenBank database (http://www.ebi.ac.uk/ena) under accession codes PRJNA292004 (Alves-Ferreira et al., 2015) and PRJNA475480 (Bruna et al., 2019) were downloaded for respective comparison. The dataset was downloaded using the tools described in: https://github.com/EnzoAndree/getENA. The seven isolates included in this study were obtained from patients from different regions of Colombia and one from Bolivia, with nodular or ulcerative cutaneous lesions. The sampling was performed before patients underwent anti-Leishmanial therapy.
Parasite Culture and Identification
The parasites obtained from six Colombian clinical isolates and the Bolivian isolate were cultured axenically in Schneider’s insect medium, which was supplemented with 10% (v/v) fetal bovine serum; the cultures were maintained at 26°C with 5% CO2 as describe previously (Patino et al., 2020). Once the parasites were in late logarithmic growth phase, were recovered by centrifugation and submitted to DNA extraction. High Pure PCR Template Preparation Kit (Roche Life Science) was used for DNA extraction in accordance with the manufacturer’s instructions. Later the concentration, quality and integrity of DNA were determined. To verify the quality of DNA, each sample was divided in two groups, the first used for species identification and the second for whole-genome sequencing.
Cytochrome b proteins (Cytb) and heat shock protein (Hsp70) were the genes selected to species identification through Sanger sequencing, as has been described previously (Ramirez et al., 2016; Patino et al., 2017). The amplification products were purified with EXOSAP (Affymetrix, USA) and sequenced using the dideoxy-terminal method in an automated capillary sequencer (AB3730; Applied Biosystem). Subsequently, sequences were subjected to a BLASTn similarity search for Leishmania sequences deposited in GenBank (Ramirez et al., 2016). Those isolates identified as L. braziliensis were selected for whole-genome sequencing.
Genomic Sequencing
HiSeq X-Ten system (Illumina) was used to sequence the whole-genome DNA, later mate-paired libraries were built and finally subjected to paired-end sequencing (2 × 150-bp read length producing a median coverage at least of 40x per sample). Reads with adapter contamination, >10% uncertain nucleotides, or >50% low-quality nucleotides (base quality < 5) were discarded (Yan et al., 2013).
DNA Mapping
The paired-end Illumina reads of six Colombian clinical isolates, one Bolivian clinical isolate and of the fourteen Brazilian genomes (data downloaded from European Nucleotide Archive) were mapped to the reference MHOMBR75/M2904_2019 L. braziliensis genome sequence assembly (http://tritrypdb.org) using the SMALT program (version 0.7.4) (www.sanger.ac.uk/resources/software/smalt/). Exhaustive search option (−x and −y of 0.8), a sliding step of 3 and a reference hash index of 13 bases were the parameters using during the mapping. To prevent the mapping of non-Leishmania reads to the reference sequences, we used an identity threshold of y = 0.8. SAMtools (version 0.1.18) and Picard (version 1.85) were used to no-mapping read exclusion, sorting, file merging and elimination of PCR duplicates (Imamura et al., 2016). The reads corresponding to the mitochondrial genome were extracted and assembled under the same pipeline described for nuclear genome but using maxicircle sequence from novel long read assembly of L. braziliensis M2904 (available in tritrypDB).
Phylogenomic Analysis
SNPs alignments from whole nuclear and mitochondrial (maxicircle) genomes unphased and phased (see “Phasing of heterozygous SNP sites” section), were used to evaluate the phylogenomic relationship among L. braziliensis isolates and between each sequence generated by each isolate during the phasing. FastTree double precision version 2.1.10. (Price et al., 2009) was used to build a based-maximum-likelihood phylogenetic tree. The robustness of the nodes was evaluated using the Bootstrap method (BT, with 1,000 replicates). The obtained tree was visualized using the interactive tool Interactive Tree Of Life V4 (http://itol.embl.de) (Letunic and Bork, 2019). To detect recombination signatures in the analyzed genomes, phylogenetic networks were built in SplitsTree5 (Huson and Bryant, 2006) using the Neighbor-Net method. We included MHOMBR75/M2904_2019 L. braziliensis as genome sequence (REF) and used as outgroup the L. guyanensis genome assembly from European Nucleotide Archive accession number SRR8179913 (Lguy_SRR8179913) (http://www.ebi.ac.uk/ena) and the L. panamensis genome assembly from TriTrypDB http://tritrypdb.org: LPPSC1 (LpW).
Somy Analysis and Gene Copy Number Variations
The chromosomal somy was estimated calculating initially the median read depth of each chromosome (di). Positions with read depth of >1 standard deviation were removed and the di recalculated. Later, we calculated the median depth (dm) of whole genome (35 chromosomes) and the somy (S-value) of each chromosome, for that we used the formula previously described S = 3 × di/dm (Patino et al., 2019a). The ranges of somy (mono-di-tri-tetra and penta somy) were defined, as previously described (Dumetz et al., 2017).
To evaluate the gene copy number variations (CNVs), we calculated and related the average haploid depth per gene without somy effect (dHG) and the full cell depth with somy effect (dFG) using the formula: (dFG = S × dHG). The statistical significance used in this study was set at a z-score cutoff of >2 and an adjusted p-value (Student’s t-test) of <0.05. Heatmaps were created using the Heatmap3 package in R (Zhao et al., 2014). Finally, we used the Gene Ontology enrichment analyses from TriTrypDB tools (http://tritrypdb.org) to evaluate the genes with CNVs. P-values were adjusted for multiple testing with Benjamini-Hochberg method with a false-discovery rate (FDR) of <0.05. The GO terms were submitted to REVIGO (Supek et al., 2011).
SNP Estimations and Analysis
Initially, the reads were mapped to the reference MHOMBR75/M2904_2019 L. braziliensis genome sequence assembly using the SMALT program (version 0.7.4), different options of this program were used to search random mapping of multiple hit reads and obtained optimal alignments. The merging and sorting of bam files and marking duplicated reads were implemented with the Picard program (version 1.85) (http://broadinstitute.github.io/picard/) as described previously (Dumetz et al., 2017). The SNPs were called with the population-based Unified Genotyper method in the Genome Analysis Toolkit (GATK) (version 3.4; https://software.broadinstitute.org/gatk/), where SNPs were called among all the samples simultaneously. Later, we realigned around indels to remove these and retrieved only the SNPs. GATK Variant Filtration was used to filter Low-quality SNPs, according to the following criteria: QD < 2.0 || MQ < 40 || FS > 60.0 || ReadPosRankSum < −8.0. The SNP quality cutoff was set at 3000. Later, the Integrative Genomic Viewer program (IGV_2_3_47) was used to visualize all SNPs identified and the SnpEff program (version v4.1) to classify the SNPs based on their functional impact (Dumetz et al., 2017). Once the SNPs were independently detected, these were extracted from nuclear and mitochondrial alignments of L. braziliensis genomes using snp-sites program (Page et al., 2016). Based on the total number of SNPs and using snp-distans program (https://github.com/tseemann/snp-dists), we generated a pairwise distance matrix; the results obtained were graphically represented. Lastly, we selected the SNPs that have a potential effect on gene function (high and moderate impact); from this selection, we identified the unique/shared SNPs between and within the clades and between the Colombian genomes, the data were included in an Excel matrix, which was used to perform the comparative analysis. Finally, to measure nucleotide diversity (π), we used the DnaSP software v.5.0.
Heterozygosity/Homozygosity Analysis
From allele frequency estimation data, we determined the Homozygous and heterozygous variants. Allele shifts of < 0.33 or > 0.66 were considered as homozygous variants while allele shifts between 0.33 and 0.66 as heterozygous variants (Tihon et al., 2017). Once homozygous and heterozygous variants were identified, these were counted using an Excel matrix. Later, the heterozygosity/homozygosity for each chromosome from each sample was extracted and calculated using the variant call formats through a Perl script. The identified heterozygous and homozygous SNPs for each chromosome per sample were plotted using the packages: Stringr, Ape, and Phangorn in R (Paradis et al., 2004; Schliep et al., 2019; Wickham, 2019).
Phasing of Heterozygous SNP Sites
Heterozygous SNPs were phased using BEAGLE v5.1 (https://faculty.washington.edu/browning/beagle/beagle.html) over 30 iterations. The algorithm also imputes missing genotypes from identity-by-state segments found in the data (Schwabl et al., 2019). A custom Perl script was used to retrieve the sequence from the phased region and replace consensus bases with SNPs, generating thus two sequences for each isolate (denote as A and B), substituting base predictions for each haplotype into each sequence. This was conducted with whole genome data and by chromosome.
Results
Identification of Leishmania Species
The Table 1 and Figure 1A describe the clinical characteristics and geographical distribution of the Colombian/Bolivian isolates analyzed in this study. The amplification and Sanger sequencing of Cytb and Hsp70 genes identified that the isolates herein included corresponded to L. braziliensis (Supplementary Table S1).
Table 1 Clinical characteristic of each Colombian clinical isolate analyzed in the study.
Figure 1 Geographical distribution of genomes included in the study and Leishmania braziliensis phylogenomic relationships among nuclear and mitochondrial genomes. (A) Geographical location of the L. braziliensis genomes analyzed. The map was built using Microreact online tool (https://microreact.org/showcase) based on the GPS coordinates of each isolation (QGIS Geographic Information System, Open Source Geospatial Foundation Project, http://qgis.osgeo.org). Phylogenomic analysis based on whole nuclear genome (B) and mitochondrial genome: maxicircle (C). MHOM/BR75/M2904_2019 L. braziliensis (REF) was included as reference genome and Lguy_SRR8179913 (L. guyanensis) and LpW (L. panamensis) were used as outgroup. Black dots represent well-supported nodes (Bootstrap ≥ 90). (D, E) represent the phylogenetic network (Neighbor-Net) constructed in SplitsTree 5, based on nuclear and mitochondrial (maxicircle) genomes respectively.
Phylogenomic Analysis
Two alignments were used to conduct phylogenomic analyses for 21 genome sequences. The first corresponded to single nucleotide polymorphisms (SNPs) from nuclear genome (1,320,593 nucleotide positions). The second corresponded to SNPs from mitochondrial genomes (167 nucleotide positions). For both cases MHOM/BR75/M2904_2019 L. braziliensis genome as the reference and L. guyanensis (Lguy_SRR8179913) and L. panamensis LP-PSC1 (LpW) genomes as outgroups. The approximately-maximum-likelihood phylogenetic trees built in FastTree double precision version 2.1.10 (Price et al., 2009) allowed initially to confirm that all the genomes evaluated were closely related to the reference genome and evidencing that the genomes analyzed here corresponding to L. braziliensis (Figures 1B, C). In addition, tree topologies (Figures 1B, C) describe the presence of four subpopulations clustered in well-supported nodes (with bootstrap ≥ 90.0). Clade 1 (highlighted in purple) included most of the Brazilian genomes (Lb1979381, Lb1980022, Lb1980024, Lb1980025, Lb7293733 Lb7293734, Lb7293735, Lb7293736, Lb7293738, Lb7293739, Lb7293740, and Lb7293741), clade 2 (highlighted in sky blue) two Colombian genomes (Lb7616 and Lb7864), clade 3 (highlighted in red) the remaining Brazilian genomes (Lb7293737 and Lb7293742), and clade 4 (highlighted in yellow) included the remaining Colombian genomes (Lb7529, Lb7740, Lb8025, and Lb8102) and the Bolivian genome (Lb7933). Interestingly, the two Colombian genomes belong to clade 2, collected from Vaupés and Antioquia were closer to the Brazilian genomes belonging to clade 3 than to genomes of clade 4, which included the other Colombian genomes collected from other regions of the country (Meta and Guajira). These findings were additionally supported by phylogenetic tree topologies obtained in SplitsTree5 (Huson and Bryant, 2006) (using neighbor-net method), where the members of these Clades were consistently clustered together and the four divergent between them (Figures 1D, E). The profiles were consistent between nuclear and mitochondrial analyses except for three remarkable events of clustering change that were defined as swapping events. These events were present in two relatively distant sub-clades within clade 4 (sub-clade 1 formed by Lb8025 and Lb7933 and sub-clade 2 by Lb7529, Lb7740, and Lb8102) (Supplementary Figure S1). Remarkably, one of the isolates that formed a possible subclade (Lb7933) was from Bolivia. Finally, we did not observe any relationship between the clustering patterns with the clinical or therapeutic characteristics of each genome analyzed.
Evaluation of Chromosome and Gene CNVs
We analyzed and compared the chromosome copy numbers in the 21 genomes included in this study. The results revealed that in some genomes the karyotype remained unchanged, most of the chromosomes presented a S value between 2.5 and 3.5, which indicate its trisomic state, with the consistent exception of chromosome 31, however, in other genomes we observed a moderate aneuploidy. In clade 1, the Lb7293740 genome presented six chromosomes (12, 18, 22, 29, 33, and 34) with extra copies, while the Lb7293733 genome presented three chromosomes (8, 26, and 27) with extra copies. Additionally, we observed that in one clade 4 genome (Lb8102), there was an extra copy of chromosome 2 compared with the other genomes of the same clade (Figure 2). We confirmed the accuracy of somy value obtained by not observing discordance in the allele frequency counts for each predicted heterozygous SNP and the value obtained between the read depths and allele frequencies.
Figure 2 Dynamic of somy between the 21 Leishmania braziliensis genomes analyzed. The heatmap shows the copy number of the 35 chromosomes (y-axis) for the 21 genomes analyzed, included the reference genome (Lb_Ref) (x-axis). Trisomic (3, blue), tetrasomic (4, green), pentasomic (5, orange).
To evaluate the genes with CNVs and compared their occurrence in the genomes analyzed, we observed that the genome with the highest number of genes with CNVs was Lb8025 (147 genes), and the genomes with the lowest numbers were Lb7293737 and Lb7293742 (83 and 80 genes, respectively) (Figure 3A). The genes that presented the highest CNVs were found in the Lb8025 genome and encoded: β-tubulin, heat shock protein 83-1 and amastin protein. The genes that exhibit CNVs in the Lb7293737 and Lb7293742 genomes were mainly associated with cell adhesion (e.g. amastin and GP63 leishmanolysin), cell transport (e.g. ABC transporter and glucose transporter 3) and cytoskeletal proteins (e.g. β-tubulin). Additionally, we observed 25 genes with CNVs that were shared between all the genomes analyzed, 22 (88%) of which had known functions and three of which (12%) were annotated as hypothetical proteins. The genes with known function encoded to heat shock protein 83 and 70, α- and β-tubulin, amastin, zinc transporter, and NADH-dependent fumarate reductase (Supplementary Table S2). Finally, the Gene Ontology enrichment analysis revealed that the genes with CNVs were mainly associated with cellular zinc ion homeostasis, response to stress, response to stimulus, and protein folding (Figure 3B).
Figure 3 Genes with copy number variation (CNV) and gene ontology enrichment analyses. (A) The bar graph represents the number of genes that presented CNVs in the 21 genomes included in the study. (B) The table represent the number of genes with CNVs in each genome analyzed, that were involved in different biological processes.
SNPs Estimations
Comparison of each genome with the reference L. braziliensis sequence revealed a significant difference in the total number of SNPs per genome in each clade analyzed. The genomes of clade 1 contain between 42,617 and 68,989 SNPs, the genomes in clade 2 contain ~ 423,200 SNPs and the genomes belonging to clades 3 and 4 contain between 104,316 and 150,639 SNPs (Supplementary Table S3). Of the total number of SNPs identified ~20% had a potential effect on gene function (high and moderate impact). The genomes with the highest number of SNPs with functional impact were Lb7616 (54,640) and Lb7864 (53,576), belonging to clade 2, followed by the clade 4 genomes (~23,000 SNPs) (Figure 4A). Considering the SNP density between the genomes analyzed, we observed that this density was variable, ranging from less than 1 SNP/Kb in genomes belonging to cluster 1, 3 and 4 to 1.7 SNPs/Kb in genomes belonging to cluster 2. The least polymorphic genomes were Lb1980024 and Lb1980025 belonging to cluster 1 (0.23 and 0.24 SNPs/Kb, respectively), and the genomes with greatest variability were the Lb7616 and Lb7864 genomes belonging to cluster 2 (1.7 SNPs/Kb) (Figure 4B). Additionally, we evaluated and compared the nucleotide diversity between Colombian and Brazilian genomes and observed a low diversity in Brazilian genomes (π = 0.072) compared with the Colombian genomes (π = 0.30).
Figure 4 Overview of SNPs identified in L. braziliensis genomes. (A) Number of SNPs per chromosome, that have a potential effect on gene function (high and moderate impact) in each of the genomes analyzed. The clade is indicated at left. (B) SNP density of 21 L. braziliensis genomes analyzed. (C) Number of SNPs unique and shared between and within the four clades identified. The colors represent each clade: clade 1 (Purple), clade 2 (sky blue), clade 3 (green) and clade 4 (light orange). (D) Summary of the number of SNPs unique and shared between the six Colombian genomes analyzed, the external square represents the unique SNPs and the inner circle the shared SNPs.
When we analyzed all genomes for SNP variants, we identify ~ 85% were synonymous variants; however, some variants had high functional impact (e.g. stop_gained and stop_lost) (Table 2). The SNPs associated to stop gained were identified in both genes encoding hypothetical proteins and in genes encoding to known function proteins such as surface proteins (amastin surface protein and phosphoglycans), multidrug resistance proteins, transporter proteins (pteridine transporter, ABC1 transporter, and iron/zinc transporter protein), and cytoskeletal proteins (β-tubulin), while the SNPs associated to stop lost were identified mainly in genes encoding hypothetical proteins and some of them in genes encoding transmembrane proteins (Meckelin transmembrane protein 67 putative) or transporter proteins (pteridine transporter) (Supplementary Table S4).
Table 2 Number of SNPs with potential effect on gene function, in each genome analyzed.
The next step was to evaluate the shared SNPs (with high and moderate functional impact) within and between each clade. The comparison of genomes within each clade revealed that the genomes of clade 2 had the highest number of shared SNPs (26,282) compared with clades 1, 3, and 4, where the number of SNPs shared was 1,173 and 9,703, and 5,520, respectively (Figure 4C). Fifty-two percent of shared SNPs in each clade were in genes encoding to hypothetical protein, and the remaining 48% were in genes encoding to proteins with known function. The comparison between the four clades allowed to identify 463 SNPs shared with known function (Figure 4C). Most of them identified in genes encoding transporter proteins (ABC transporter, nucleoside transporter, zinc transporter, and sugar transporter), host-pathogen interaction-associated proteins (amastin surface protein), kinetoplast-associated protein, or intracellular degradation-associated proteins (ubiquitin-conjugating enzyme putative) (Supplementary Table S5). Regarding the shared SNPs between the clades, we observed a high number of shared SNPs between clade 2 and clade 3 (5,095) followed by the clades 2 and 4 (3,591) and clades 3 and 4 (3,145); additionally, we observed that the genomes belonging to clade 1 presented a low number of shared SNPs with the other clades (clades 1 and 2: 669; clades 1 and 3: 689; clades 1 and 4: 692) (Figure 4C).
Lastly, we evaluated the unique/shared SNPs (with high and moderate functional impact) between the Colombian genomes included in the study. To analyze the unique SNPs in each genome, we observed that Lb7864 and Lb7616 were the genomes with the lowest number of SNPs (3 and 181, respectively), which represent less than 1% of the total, compared with the other genomes analyzed where the number of unique SNPs ranged between 9 and 13% (Figure 4D). Regarding the shared SNPs, the results revealed 7,512 SNPs between the genomes analyzed, 48% of them in genes encoding proteins associated with hypothetical function and the remaining 52% in genes with known function (Figure 4D). Later, we made a pairwise comparison considering not only the SNPs with high and moderate functional impact but also those located in genes with known function. The results revealed that the genomes with the highest number of shared SNPs between them were the genomes Lb7864 and Lb7616 (26,763 SNPs), followed by the genomes Lb7529, Lb8025, and Lb7740, which presented a number of shared SNPs ranging from 7,700 to 7,897 (Supplementary Table S6).
LOH and Hybridization in L. braziliensis
Finally, of the SNPs with high and moderate functional impact, 73% were homozygous and 27% heterozygous. The most interesting result was the whole genome loss of heterozygosity (LOH) observed in some genomes (Figure 5). One Colombian genome (Lb7864) and two Brazilian genomes (Lb7293737 and Lb7293742) presented LOH in all 35 chromosomes (Supplementary Figure S2 and Supplementary Table S7). To explain graphically these results, we selected one of the largest chromosomes comprising the L. braziliensis genome (Chromosome 35) and compared the heterozygosity/homozygosity (blue/green, respectively) along this chromosome among seven genomes (Figure 5). Figures 5A–C represent the Brazilian genomes and clearly show the absence of heterozygous SNPs (blue color) along chromosome 35 in the Lb7293737 and Lb7293742 genomes compared with Lb7293738 genome. Panels D–F represent Colombian genomes and show the high levels of homozygous SNPs (green color) in the Lb7616 and Lb7864 genomes (Figure 5).
Figure 5 Homozygosity/heterozygosity profile of chromosome 35 in L. braziliensis Colombian/Brazilian genomes. The distribution of homozygous (green color) and heterozygous (blue color) SNPs along chromosome 35. The X axis represents 10 kb windows of chromosome 35 and the Y axis indicates the total number of SNPs. Each panel represents a different genome; (A–C) represent the Brazilian genomes and (D–G) the Colombian genomes.
In contrast, we identified one genome of clade 4 (Lb8025) to be highly heterozygous throughout the genome (99% heterozygous SNPs) (Supplementary Table S7). This finding, which was consistent in all chromosomes (Figure 5G and Supplementary Figure S2), together with the divergence observed when compared with the others belonging to the same clade (Supplementary Figure S3), suggest a possible event of hybridization. With the purpose to analyze this possible event, we selected the genomes closely related to Lb8025 (Lb7933, Lb8102, Lb7529, and Lb7740) (Figure 1) and reconstructed haplotypes of each of them from both nuclear and mitochondrial genomes and by chromosome through phasing the genotype calls. The results obtained when analyzing the topologies of trees from the nuclear (Figure 6A) and mitochondrial genomes (Figure 6B) and the results obtained by chromosome (Supplementary Figure S4) showed that across the genome, the haplotype A and the haplotype B of Lb8025 (Lb8025-A and Lb8025-B) are grouped in distinct clusters, which contrast with the observed in the other genomes analyzed. This is a strong genomic evidence of hybridization with alleles from independent ancestries.
Figure 6 Phylogenetic reconstruction based on genomic SNP alignments for phased haplotypes belonging to Clade 4. The trees represent phylogenetic analysis from whole nuclear (A) and mitochondrial (maxicircle) (B) single-nucleotide polymorphism (SNP) alignments based on phased haplotypes of five genome sequences belonging clade 4 (highlighted in yellow). MHOM/BR75/M2904_2019 L. braziliensis (REF) was included as reference genome. Black dots represent well-supported nodes (Bootstrap ≥ 90) and the dotted squares show the location of Lb8025 haplotypes.
Discussion
To understand the genetic heterogeneity of L. braziliensis, we used DNA-seq technology and bioinformatic analyses to investigate the genetic structure and the phylogenomic relationships among L. braziliensis genomes from Colombian/Bolivian clinical isolates and among genomes of Brazilian L. braziliensis isolates archived in public databases. The phylogenetic analysis of nuclear and mitochondrial genomes revealed the presence of two different L. braziliensis populations in Colombia (clade 2 and clade 4) (Figures 1B–E). We considered that the presence of these clades within Colombia can be associated with (i): the geographical localization of each clade; clade 4 genomes are located outside of mountain ecosystems (east Andes), while the clade 2 genome (Lb7864) occurs in Colombian mountain ecosystems (central cordillera) (Figure 1A), indicating that the Andes might generate intra-species diversification, as has been proposed for different arthropods, such as bees (Dick et al., 2004), butterflies (De-Silva et al., 2016; Chazot et al., 2018), arachnids (Salgado-Roa et al., 2018), Triatominae (Gomez-Palacio and Triana, 2014; Monsalve et al., 2016), Lutzomyia species (Gonzalez et al., 2014; Ferro et al., 2015) and plants, such as Phlegmariurus (Testo et al., 2019). (ii): Constant human displacement due to violence, armed conflict, or the deployment of military troops from areas of high endemicity (Ore et al., 2015). This could promote the distribution of this parasite to other geographical regions, as has been observed for L. guyanensis, which broke out of its native geographical distribution and is now detected in two different habitats (Ferro et al., 2011; Ramirez et al., 2016). Finally, (iii): migration of animal reservoirs or sand fly vectors responsible for transmission of the parasite within the national territory. L. braziliensis is associated with a wide variety of sand fly vectors (Bejarano et al., 2002; Testa et al., 2002; Ovallos et al., 2013; Ovalle-Bracho et al., 2019).
Another interesting finding was associated with the clear swapping event (incongruence in the tree topologies) identified in clade 4, when tree topology of nuclear genome was compared with the mitochondrial genome (Figure 1 and Supplementary Figure S1). This finding was mainly observed in the Lb8025 genome, which may represent evidence of introgression, probably by interspecific hybridization, phenomena that has been described as a possible way for rapid evolution, which is key in species responses to environmental change or as a strategy of parasites to escape from Muller’s ratchet (irreversible accumulation of deleterious mutations) (Harrison and Larson, 2014; Messenger and Miles, 2015). This was expected because of recent evidence of hybridization and meiosis-like behavior in Leishmania and other trypanosomatids, such as Trypanosoma cruzi (Inbar et al., 2019; Schwabl et al., 2019; Broeck et al., 2020). We were able to identify a potential hybrid; however, considered that additional studies are necessary to understand not only the population structure but also to describe the evolutionary forces that possibly have promoted it. Therefore, studies that involved a larger population size from different countries, as well as crosses in sandflies should be performed.
When we then evaluated the somy value; observed that the Brazilian genomes had high karyotype instability compared with the Colombian/Bolivian genomes, of which only two of the seven genomes showed somy change (Lb8102 and Lb8025 genomes) (Figure 2). Aneuploidy is considered one of the most important processes for Leishmania adaptation; therefore, these results indicate that Colombian strains could be changing to adapt to the eco-epidemiological conditions of the country as the Brazilian strains and that the amplification of some chromosomes may provide fitness advantages during host adaptation, which is consistent with the observed in other Leishmania species (L. donovani, L. major, L. tropica, L. infantum, and L. amazonensis) (Dumetz et al., 2017; Iantorno et al., 2017; Bussotti et al., 2018; Patino et al., 2019b). Additionally, the somy dynamic observed reveals changes in an individualized manner, suggesting that environment-independent intrinsic genetic factors can affect Leishmania karyotypic adaptation, as has been described in Old-Word Leishmania species (Bussotti et al., 2018). Despite moderate karyotype heterogeneity observed in the Colombian/Bolivian genomes, we highlight an interesting somy dynamic observed in the Lb8025 genome (Figure 2), in which the genetic content was doubled in various chromosomes. This, together with the possible introgression event (Figure 1 and Supplementary Figure S1), the elevated heterozygosity (Figure 5G, Supplementary Figure S2, and Supplementary Table S7), the divergence compared with other genomes of the same clade (Supplementary Figure S3), and the grouping of each haplotype in two different clusters (Figure 6 and Supplementary Figure S4) indicate that this strain is a hybrid (via intra or interspecific hybridization or genomic recombination). The genetic exchange product of hybridization event could generate evolutionary advantages that can impact vector permissiveness, the adaptation to different environments/niches, to increase or alter the virulence and generate recombinant progeny that are capable of widespread clonal propagation. Likewise, the pathological implications of hybrid genotypes in human infections, could promote the spread of virulent strains to affect the transmissibility and the resistance to chemotherapeutics (Cortes et al., 2012; Ramirez et al., 2012; Messenger and Miles, 2015).
Another evaluated parameter was local copy number variation (CNV). The results did not reveal important intraspecies variations in the numbers of genes with CNV (Figure 3A) either in genomes collected within or outside of Colombia. Of the genes showing CNV in all analyzed genomes (25 in total), we highlight those involved in stress resistance, infectivity and virulence (Heat shock protein 70 and 83, α and β tubulin, amastin, and zinc transporter) (Supplementary Table S2); curiously, these genes were previously reported in other studies (Rastrojo et al., 2013; Bifeld and Clos, 2015; Bussotti et al., 2018; Urrea et al., 2018; Restrepo et al., 2019). These results together with the ontology enrichment analysis (Figure 3B), indicate that these genes may drive or be the result of rapid adaptation in L. braziliensis, results previously described in other Leishmania species (Rogers et al., 2011; Bussotti et al., 2018; Urrea et al., 2018) and suggest that genome plasticity in L. braziliensis not only at the level of whole chromosome but also of specific regions promotes important processes associated with the replication, infectivity, and virulence.
Likewise, the local copy number variation may represent an evolutionarily important adaptation, to confer a degree of plasticity in the regulation and functional expression of genes or gene clusters involved in drug resistance, adaptation to different environmental, including survival in a variety of mammalian hosts and phenotypic diversity as has been observed in other species (L. donovani, L. tropica, L. infantum, and L. panamensis) (Laffitte et al., 2016; Iantorno et al., 2017; Sinha et al., 2018; Patino et al., 2020).
Our final strategy to evaluate genomic variability among L. braziliensis genomes involved analysis of nucleotide-level variations (SNPs). Comparing the number of SNPs found in this study (42,617 to 435,529; n = 21) (Supplementary Table S3) and the recent report in L. braziliensis isolates from Peru (Broeck et al., 2020), with the number of variants described in other Old/New World Leishmania species, such as L. donovani (3,549 SNPs; n = 17) (Downing et al., 2011); L. infantum (17,333 SNPs; n = 12) (Rogers et al., 2014) (~3,000 SNPs; n = 20) (Teixeira et al., 2017), L. amazonensis/L. mexicana vs. L. infantum (~21,000 SNPs; n = 2) (Valdivia et al., 2017), L. panamensis; (~62,000 SNPs; n = 22) (Patino et al., 2020), L. amazonensis vs. reference genome of L. mexicana (~40,000 SNPs) (Patino et al., 2019b) and L. peruviana vs. reference genome of L. braziliensis (~112,000 SNPs; n = 2) (Valdivia et al., 2015), we can confirm that L. braziliensis, is the Leishmania (Viannia) species with the highest genetic variability circulating in some regions of South America (Brazil, Colombia and Perú). Additionally, to analyze the genetic variability of L. braziliensis between the Colombian and Brazilian isolates, we observed that Colombian genomes were much more diverse (π = 0.30; n = 7), than the Brazilian genomes (π = 0.072; n = 14) (Supplementary Table S3). This diversity is advantageous for the parasite because it favors survival in diverse ecological systems/niches, determines the distribution of the observed clinical forms of the disease, and influences the anti-leishmanial therapy response; aspects that enable this species to be a successful human pathogen. Despite our results, we consider that additional studies, with a greater number of Colombian samples, are necessary to understand the reasons why L. braziliensis is such a highly diverse species.
To evaluate unique/shared SNPs among the four identified clades, we identified a high number of unique SNPs (Figure 4C), mainly in the clades with low heterozygosity (Figure 5 and Supplementary Table S7) (genomes belonging to clades 2 and 3). Consistently, we observed a low number of shared SNPs among the four clades (463 SNPs) (Figure 4C). The analysis of shared SNPs between clades revealed the low similarity between the genomes belonging to clade 1 and the genomes belonging to other clades, contrary to observed with the genomes of clades 2, 3, and 4, which presented a high similarity between them (high number of shared SNPs), those results confirm the close relationship between the Colombian genomes with some Brazilian genomes (Figure 4C). Finally, we analyzed the unique/shared SNPs, but in this case between the six Colombian genomes. The results revealed in some genomes a low number of unique SNPs and a high number of shared SNPs (Figure 4D; Supplementary Table S6); two of the genomes (Lb7864 and Lb7616) had 99.5% of identity, and this close inter-strain relationship could indicate a recent shared ancestor between them.
In addition to the findings associated with the elevated number of SNPs in the genomes herein analyzed, we highlight the drastic whole genome loss of heterozygosity (LOH) observed in some Brazilian/Colombian genomes (specifically in those belonging to clades 2 and 3) (Figure 5 and Supplementary Table S7). This large-scale of LOH, has been observed in fungi (Candida albicans and Saccharomyces cerevisiae) as a mechanism for introducing diversity into a population and as a strategy to survive in stressful conditions (response to treatment, DNA damage and host passage) (Dunkel and Morschhauser, 2011; Hirakawa et al., 2015; Wertheimer et al., 2016; Hoffert and Strome, 2019). In Leishmania, studies published to date, reveal LOH to short scale (loss of some heterozygous SNPs in a block) (Samarasinghe et al., 2018) or low-level heterozygosity (Downing et al., 2011; Rogers et al., 2014) mainly in Old World Leishmania parasites (L. infantum, L. major and L. donovani). Therefore, this is the first study using Colombian clinical isolates and next generation sequencing to identify patterns of homozygosity in the whole genome of one of the most important New World Leishmania species, L. braziliensis. This pattern of diversity could be explained by a substantial bottleneck of adaptation that occurred in an environment/niche-dependent manner, which favored and fixed certain genotypes in the population and promoted the stochastic loss of others, as has been described for T. brucei (Oberle et al., 2010) and recently for T. terrestris (Perez et al., 2019). Another possible hypothesis for these findings is associated with gene conversion, which may have produced unidirectional transfer of genetic material between members of the same species sharing the same ecological niche. This would contribute to the genetic diversity and possible evolutionary success of the species, as has been suggested for this and other Leishmania species (L. donovani complex) (Mauricio et al., 2007; Rougeron et al., 2009), as well as other eukaryotic organisms (Theileria parva and Saccharomyces cerevisiae) (Qi et al., 2009; Henson et al., 2012; Sriswasdi et al., 2016). The structure of the L. braziliensis genome is complex, and additional studies are needed to unveil the existence of homozygous and heterozygous strains and the exact mechanism by which Leishmania can fix its heterozygosity including the biological consequences of this plasticity.
In conclusion, the findings in this study demonstrate the high intra-species genetic diversity of L. braziliensis in Colombia and the occurrence of distinct phylogenetic groups in the sampled regions. The genomic changes, until date unknown in L. braziliensis, such as moderate changes in somy, CNVs, the introgression event, the increase of the heterozygosity and the duplication of genetic content in various chromosomes, identified in the Lb8025 genome (Potential hybrid), as well as the high number of homozygous SNPs (most of them unique for each clade) and the whole genome LOH, identified in Colombian/Brazilian genomes, suggest a striking genomic plasticity of this species, which could be genomic strategies used by L. braziliensis to favor its survival and adaptation to different ecological niches. These genomic findings can influence L. braziliensis epidemiology and future clinical and therapeutic outcomes.
Although our study was focused to analyze and compare the genomic structure of Colombian/Bolivian isolates with Brazilian isolates, we consider that there are necessary additional studies that include genomes from other regions of South America where L. braziliensis is endemic. This at the end will permit not only to make a broader comparative genomic analysis of our Colombian isolates with genomes from other regions but also to expand the knowledge about of genetic variability of this species in South America.
Data Availability Statement
The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found at: https://www.ebi.ac.uk/ena, ERS4385933, ERS4385934, ERS4385935, ERS4385936, ERS4385937, ERS4385938 and ERS4385939.
Ethics Statement
The studies involving human participants were reviewed and approved by: This study was approved by the Ethics Committee of the Universidad de Antioquia (number VRI3445/2010) in accordance with resolution number 36836. Written informed consent was obtained from the patients from which the strains were isolated. The patients/participants provided their written informed consent to participate in this study.
Author Contributions
LP conceived and designed the study, analyzed and interpreted the data, and prepared the manuscript. MM interpreted the data and critically revised the manuscript and made important suggestions. LC-S critically revised the manuscript and made important suggestions. CM critically revised the manuscript and made important suggestions. JR conceived and designed the study and revised the manuscript. All authors contributed to the article and approved the submitted version.
Funding
This work was funded by DIRECCIÓN DE INVESTIGACIÓN E INNOVACIÓN from Universidad del Rosario. JR is a Latin American fellow in Biomedical Sciences, supported by The Pew Charitable Trusts.
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Acknowledgments
We thank the Colombian Science, Technology, and Innovation Ministry (MinCiencias) for sponsoring PhD training in Colombia, within the framework of the National Program for Promoting Research Training (sponsorship calls 647). We are also grateful to the Programa de Control y Estudio de Enfermedades Tropicales (PECET) for assistance with sampling and to Dr. Hideo Imamura for assistance with the analyses. We thank the High Computing Cluster (CENTAURO) Service from Universidad del Rosario. We thank Jeremy Allen, PhD, from Edanz Group (www.edanzediting.com/ac) for editing a draft of this manuscript.
Supplementary Material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2020.582192/full#supplementary-material
Supplementary Figure 1 | Tree comparisson of phylogenetic reconstruction from nuclear and mitochondrial alignments for Leishmania braziliensis genomes. Phylogenetic tree built based on a distance matrix, derived from nuclear SNPs (A) and mitochondrial (maxicircle) SNPs (B), shared among 21 clinical isolates of L. braziliensis. Tree topologies were compared to identify swapping events, characterized by changes in clustering patterns. Colors indicate similarity to most common node. A score of 1 denotes the subtree structure of the node is identical to the subtree structure of its best corresponding node. This comparison was performed using Phylo.io interactive tool (http://phylo.io/). Lguy_SRR8179913 (L. guyanensis) and LpW (L. panamensis) were used as outgroup and MHOM/BR75/M2904_2019 L. braziliensis (REF) as reference genome.
Supplementary Figure 2 | Homozygosity/heterozygosity profile to all 35 chromosomes in L braziliensis Colombian/Brazilian genomes. The figure represents the distribution of homozygous (green color) and heterozygous (blue color) SNPs along chromosome. The X axis represents 10 kb windows of chromosome and the Y axis indicates the total number of SNPs. Each panel represents a different genome.
Supplementary Figure 3 | Phylogenetic network based on nuclear SNPs alignments for the five genomes belonging Cluster-4. Neighbour-joining network based on genome-wide SNPs for the five genomes belonging clade 4. MHOM/BR75/M2904_2019 L. braziliensis (REF) was used as reference genome. The network was constructed using SplitsTree 5.
Supplementary Figure 4 | Phylogenetic reconstruction based on genomic SNP variation of phased haplotypes belonging Clade 4 per chromosome. The trees represent phylogenetic analysis, per chromosome, of nuclear single-nucleotide polymorphism (SNP) alignments based on phased haplotypes of five genome sequences belonging clade 4 (highlighted in yellow). MHOM/BR75/M2904_2019 L. braziliensis (REF) was included as reference genome. Black dots represent well-supported nodes (Bootstrap ≥ 90) and the dotted squares show the ubication of Lb8025 haplotypes.
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Keywords: DNA-seq, genomic variability, copy number variations, single-nucleotide polymorphisms, Leishmania braziliensis
Citation: Patino LH, Muñoz M, Cruz-Saavedra L, Muskus C and Ramírez JD (2020) Genomic Diversification, Structural Plasticity, and Hybridization in Leishmania (Viannia) braziliensis. Front. Cell. Infect. Microbiol. 10:582192. doi: 10.3389/fcimb.2020.582192
Received: 10 July 2020; Accepted: 28 September 2020;
Published: 16 October 2020.
Edited by:
Herbert Leonel de Matos Guedes, Federal University of Rio de Janeiro, BrazilReviewed by:
Jose M. Requena, Autonomous University of Madrid, SpainIsabel Mauricio, New University of Lisbon, Portugal
Sarah Forrester, University of York, United Kingdom
Copyright © 2020 Patino, Muñoz, Cruz-Saavedra, Muskus and Ramírez. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Juan David Ramírez, juand.ramirez@urosario.edu.co