AUTHOR=Dinh Truc Thi Huong , Tummamunkong Phawida , Padungros Panuwat , Ponpakdee Pranpariya , Boonprakong Lawan , Saisorn Wilasinee , Leelahavanichkul Asada , Kueanjinda Patipark , Ritprajak Patcharee 

TITLE=Interaction Between Dendritic Cells and Candida krusei β-Glucan Partially Depends on Dectin-1 and It Promotes High IL-10 Production by T Cells

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=10

YEAR=2021

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2020.566661

DOI=10.3389/fcimb.2020.566661

ISSN=2235-2988

ABSTRACT=<p>Host-<italic>Candida</italic> interaction has been broadly studied during <italic>Candida albicans</italic> infection, with a progressive shift in focus toward non-<italic>albicans Candida</italic> species. <italic>C. krusei</italic> is an emerging multidrug resistant pathogen causing rising morbidity and mortality worldwide. Therefore, understanding the interplay between the host immune system and <italic>C. krusei</italic> is critically important. <italic>Candia</italic> cell wall β-glucans play significant roles in the induction of host protective immune responses. However, it remains unclear how <italic>C. krusei</italic> β-glucan impacts dendritic cell (DC) responses. In this study, we investigated DC maturation and function in response to β-glucans isolated from the cell walls of <italic>C. albicans</italic>, <italic>C. tropicalis</italic>, and <italic>C. krusei</italic>. These three distinct <italic>Candida</italic> β-glucans had differential effects on expression of the DC marker, CD11c, and on DC maturation. Furthermore, bone-marrow derived DCs (BMDCs) showed enhanced cytokine responses characterized by substantial interleukin (IL)-10 production following <italic>C. krusei</italic> β-glucan stimulation. BMDCs stimulated with <italic>C. krusei</italic> β-glucan augmented IL-10 production by T cells in tandem with increased IL-10 production by BMDCs. Inhibition of dectin-1 ligation demonstrated that the interactions between dectin-1 on DCs and cell wall β-glucans varied depending on the <italic>Candida</italic> species. The effects of <italic>C. krusei</italic> β-glucan were partially dependent on dectin-1, and this dependence, in part, led to distinct DC responses. Our study provides new insights into immune regulation by <italic>C. krusei</italic> cell wall components. These data may be of use in the development of new clinical approaches for treatment of patients with <italic>C. krusei</italic> infection.</p>