AUTHOR=Valverde-Villegas Jacqueline María , Naranjo-Gomez Mar , Durand Mélusine , Rutagwera David , Bedin Anne-Sophie , Kankasa Chipepo , Debiesse Ségolène , Nagot Nicolas , Tuaillon Edouard , Van de Perre Philippe , Molès Jean-Pierre TITLE=The CD133+ Stem/Progenitor-Like Cell Subset Is Increased in Human Milk and Peripheral Blood of HIV-Positive Women JOURNAL=Frontiers in Cellular and Infection Microbiology VOLUME=10 YEAR=2020 URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2020.546189 DOI=10.3389/fcimb.2020.546189 ISSN=2235-2988 ABSTRACT=

Human milk is a significant source of different CD133⁺ and/or CD34⁺ stem/progenitor-like cell subsets in healthy women but their cell distribution and percentages in this compartment of HIV-positive women have not been explored. To date, a decrease of CD34⁺ hematopoietic stem and progenitor cell frequencies in peripheral blood and bone marrow of HIV-positive patients has been reported. Herein, human milk and peripheral blood samples were collected between day 2–15 post-partum from HIV-positive and HIV-negative women, and cells were stained with stem cell markers and analyzed by flow cytometry. We report that the median percentage of CD45^+/highCD34⁻CD133⁺ cell subset from milk and blood was significantly higher in HIV-positive than in HIV-negative women. The percentage of CD45^dimCD34⁻CD133⁺ cell subset from blood was significantly higher in HIV-positive than HIV-negative women. Moreover, percentages of CD45^dimCD34⁺, CD45^dimCD34⁺CD133⁻, and CD45^+highCD34⁺CD133⁻ cell subsets from blood were significantly lower in HIV-positive than HIV-negative women. The CD133⁺ stem/progenitor-like cell subsets are increased in early human milk and blood of HIV-positive women and are differentially distributed to CD34⁺ cell subset frequencies which are decreased in blood.