AUTHOR=Chen Lili , Li Shue , Nie Jiaming , Zhao Jiajia , Yu Shaoling , Li Yaoxu , Peng Jinfeng 

TITLE=Bmal1 Regulates Coagulation Factor Biosynthesis in Mouse Liver in Streptococcus oralis Infection

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=10

YEAR=2020

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2020.530190

DOI=10.3389/fcimb.2020.530190

ISSN=2235-2988

ABSTRACT=<p><italic>Streptococcus oralis (S. oralis</italic>) has been recognized as a fatal pathogen to cause multiorgan failure by contributing to the formation of microthrombus. Coagulation and fibrinolysis systems have been found under the control of circadian clock genes. This study aimed to explore the correlation between <italic>BMAL1</italic> and coagulation factor biosynthesis in <italic>S. oralis</italic> infection. Mice were administered <italic>S. oralis</italic> to induce sepsis, and HepG2 cells were also infected by <italic>S. oralis</italic>. The expression of <italic>BMAL1</italic> of hepatocytes was downregulated in the <italic>S. oralis</italic> infection group, leading to the downregulation of coagulation factor VII (FVII) and the upregulation of the coagulation factor XII (FXII) <italic>in vitro</italic> and <italic>in vivo</italic>. Furthermore, we confirmed that the deficiency of <italic>BAML1</italic> contributed to the elevation of FVII and the decline in FXII by constructing BMAL1-deficiency (<italic>Bmal1</italic><sup>−/−</sup>) mice. The current result showed that BMAL1 regulates FVII directly. Thus, a novel insight into the coagulation abnormality in <italic>S. oralis</italic> infection was gained that may optimize the treatment of sepsis by rescuing the expression of BMAL1 in the liver.</p>