AUTHOR=Wang Kaiying , Li Peihan , Lin Yanfeng , Chen Hongbin , Yang Lang , Li Jinhui , Zhang Tingyan , Chen Qichao , Li Zhonghong , Du Xinying , Zhou Yusen , Li Peng , Wang Hui , Song Hongbin TITLE=Metagenomic Diagnosis for a Culture-Negative Sample From a Patient With Severe Pneumonia by Nanopore and Next-Generation Sequencing JOURNAL=Frontiers in Cellular and Infection Microbiology VOLUME=10 YEAR=2020 URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2020.00182 DOI=10.3389/fcimb.2020.00182 ISSN=2235-2988 ABSTRACT=
Rapid and accurate etiologic diagnosis accelerates targeted antimicrobial therapy. Metagenomic analysis has played a critical role in pathogen identification. In this study, we leveraged the advantages of both the MinION and BGISEQ-500 platforms to make a bacteriologic diagnosis from a culture-negative lung tissue sample from an immunocompromised patient with severe pneumonia. Real-time nanopore sequencing rapidly identified