AUTHOR=Mouyna Isabelle , Dellière Sarah , Beauvais Anne , Gravelat Fabrice , Snarr Brendan , Lehoux Mélanie , Zacharias Caitlin , Sun Yan , de Jesus Carrion Steven , Pearlman Eric , Sheppard Donald C. , Latgé Jean-Paul 

TITLE=What Are the Functions of Chitin Deacetylases in Aspergillus fumigatus?

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=10

YEAR=2020

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2020.00028

DOI=10.3389/fcimb.2020.00028

ISSN=2235-2988

ABSTRACT=<p>Deacetylation of chitin by chitin deacetylases (Cda) results in the formation of chitosan. Chitosan, a polymer of β1,4 linked glucosamine, plays multiple roles in the function of the fungal cell wall, including virulence and evasion of host immune responses. In this study, the roles of chitosan and putative <italic>CDA</italic>s in cell wall structure and virulence of <italic>Aspergillus fumigatus</italic> were investigated. Low levels of chitosan were found in the conidial and cell wall of <italic>A. fumigatus</italic>. Seven putative <italic>CDA</italic> genes were identified, disrupted and the phenotype of the single mutants and the septuple mutants were investigated. No alterations in fungal cell wall chitosan levels, changes in fungal growth or alterations in virulence were detected in the single or septuple Δ<italic>cda1-7</italic> mutant strains. Collectively, these results suggest that chitosan is a minority component of the <italic>A. fumigatus</italic> cell wall, and that the seven candidate Cda proteins do not play major roles in fungal cell wall synthesis or virulence. However, Cda2 is involved in conidiation, suggesting that this enzyme may play a role in N-acetyl-glucosamine metabolism.</p>