AUTHOR=Weng Shan-Shan , Zhang Han-Yue , Ai Jing-Wen , Gao Yan , Liu Yuan-Yuan , Xu Bin , Zhang Wen-Hong 

TITLE=Rapid Detection of Nocardia by Next-Generation Sequencing

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=10

YEAR=2020

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2020.00013

DOI=10.3389/fcimb.2020.00013

ISSN=2235-2988

ABSTRACT=<p>In this original study, we retrospectively reviewed the cases of nocardiosis diagnosed through culture and next-generation sequencing (NGS) methods between 2014 and 2018 in Huashan Hospital and found out that the latter way can not only improve the detection rate of <italic>Nocardia</italic> spp. but also greatly reduce the turnaround time. In addition, by comparing nocardiosis and non-nocardiosis patients both of whose samples had <italic>Nocardia</italic> spp. detected by NGS, we found that <italic>Nocardia</italic>'s specific reads ranking among top two might be a satisfactory cutoff value for clinical diagnosis of the disease. Our study introduced the promising value of the NGS method in the rapid diagnosis of nocardiosis.</p>