AUTHOR=Lian Xinlei , Wang Xiran , Liu Xiao , Xia Jing , Fang Liangxing , Sun Jian , Liao Xiaoping , Liu Yahong 

TITLE=oqxAB-Positive IncHI2 Plasmid pHXY0908 Increase Salmonella enterica Serotype Typhimurium Strains Tolerance to Ciprofloxacin

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=9

YEAR=2019

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2019.00242

DOI=10.3389/fcimb.2019.00242

ISSN=2235-2988

ABSTRACT=<p><italic>Salmonella enterica</italic> serotype Typhimurium is a major global food-borne pathogen and causes life-threatening infections. Although the resistance mechanisms to fluoroquinolones in <italic>S</italic>. Typhimurium had been well-defined, tolerance to fluoroquinolones and the associated mechanism for this are obscure. In the current work, we investigated an <italic>oqxAB</italic>-positive plasmid pHXY0908 and analyzed its role in <italic>S</italic>. Typhimurium tolerance to ciprofloxacin using time-kill, transcriptome sequencing and real-time PCR. <italic>S</italic>. Typhimurium ATCC14028 could survive under lethal concentrations of ciprofloxacin after acquiring plasmid pHXY0908. Transcriptome sequence analysis showed the chromosomal genes were systematically regulated after acquiring this plasmid suggesting an interaction between chromosome and plasmid. Additionally, the chromosomal efflux pump genes <italic>acrB, acrA, tolC</italic>, and <italic>yceE</italic> were up-regulated after acquiring plasmid pHXY0908 suggesting that these efflux pumps may contribute to the survival of ATCC14028 exposed to the lethal concentrations of ciprofloxacin. In conclusion, this is the first known report demonstrating that an IncHI2 type plasmid harboring <italic>oqxAB</italic> could assist <italic>S</italic>. Typhimurium survival under lethal concentrations of ciprofloxacin.</p>