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CORRECTION article

Front. Cell. Infect. Microbiol., 11 December 2018
Sec. Microbiome in Health and Disease
This article is part of the Research Topic Interplay of Infection and Microbiome View all 13 articles

Corrigendum: Profiling the Urinary Microbiota in Male Patients With Bladder Cancer in China

  • 1Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, China
  • 2School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, China

A Corrigendum on
Profiling the Urinary Microbiota in Male Patients With Bladder Cancer in China

by Wu, P., Zhang, G., Zhao, J., Chen, J., Chen, Y., Huang, W., et al. (2018). Front. Cell. Infect. Microbiol. 8:167. doi: 10.3389/fcimb.2018.00167

In the original article, there was an error. Readers were unable to access the 16S sequencing data submitted to the Short Read Archive (SRA) under the accession number SUB3915640.

A correction has been made to MATERIALS AND METHODS, DNA Isolation and 16S rRNA Gene Sequencing:

“To avoid contamination, DNA isolation was performed using the cultured cells protocol supplied with the DNeasy Blood and Tissue Kit (Qiagen, Germany) in a laminar flow hood. The concentration of extracted DNA was determined through a Nanodrop ND-1000 spectrophotometer (Thermo Electron Corporation, USA). The genomic DNA isolated from the clinical samples was amplified using primer sets specific for V4 regions (515F: GTGCCAGCMGCCGCGGTAA; and 806R:GGACTACHVGGGTWTCTAAT). In order to evaluate contribution of extraneous DNA from reagents, extraction negative controls (no urine) and PCR negative controls (no template) were included. The resultant PCR products were purified by Qiaquick PCR purification kit (Qiagen, Valencia, CA). Finally, purified samples were normalized to equal DNA concentration and sequenced using the Illumina Miseq sequencer (Illumina, Inc., USA). The 16S rRNA gene sequences have been submitted to the Short Read Archive (SRA) under accession number PRJNA486651”.

The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

Keywords: urinary bladder neoplasms, urinary tract, microbiota, extracellular matrix, inflammation

Citation: Wu P, Zhang G, Zhao J, Chen J, Chen Y, Huang W, Zhong J and Zeng J (2018) Corrigendum: Profiling the Urinary Microbiota in Male Patients With Bladder Cancer in China. Front. Cell. Infect. Microbiol. 8:429. doi: 10.3389/fcimb.2018.00429

Received: 29 November 2018; Accepted: 29 November 2018;
Published: 11 December 2018.

Approved by:

Frontiers in Cellular and Infection Microbiology Editorial Office, Frontiers Media SA, Switzerland

Copyright © 2018 Wu, Zhang, Zhao, Chen, Chen, Huang, Zhong and Zeng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Peng Wu, doctorwupeng@gmail.com

These authors have contributed equally to this work

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