AUTHOR=He Yang , Wang Mingshu , Liu Mafeng , Huang Li , Liu Chaoyue , Zhang Xin , Yi Haibo , Cheng Anchun , Zhu Dekang , Yang Qiao , Wu Ying , Zhao Xinxin , Chen Shun , Jia Renyong , Zhang Shaqiu , Liu Yunya , Yu Yanling , Zhang Ling TITLE=Cas1 and Cas2 From the Type II-C CRISPR-Cas System of Riemerella anatipestifer Are Required for Spacer Acquisition JOURNAL=Frontiers in Cellular and Infection Microbiology VOLUME=8 YEAR=2018 URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2018.00195 DOI=10.3389/fcimb.2018.00195 ISSN=2235-2988 ABSTRACT=
Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins provide acquired genetic immunity against the entry of mobile genetic elements (MGEs). The immune defense provided by various subtypes of the CRISPR-Cas system has been confirmed and is closely associated with the formation of immunological memory in CRISPR arrays, called CRISPR adaptation or spacer acquisition. However, whether type II-C CRISPR-Cas systems are also involved in spacer acquisition remains largely unknown. This study explores and provides some definitive evidence regarding spacer acquisition of the type II-C CRISPR-Cas system from