AUTHOR=Chunfa Liu , Xin Sun , Qiang Li , Sreevatsan Srinand , Yang Lifeng , Zhao Deming , Zhou Xiangmei 

TITLE=The Central Role of IFI204 in IFN-β Release and Autophagy Activation during Mycobacterium bovis Infection

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=7

YEAR=2017

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2017.00169

DOI=10.3389/fcimb.2017.00169

ISSN=2235-2988

ABSTRACT=<p><italic>Mycobacterium bovis</italic> (<italic>M. bovis</italic>) is the pathogen of animals and humans that can replicate in the phagosomes of myeloid cells. Cytosolic detection of bacterial products plays a crucial role in initiating the innate immune response, including autophagy activation and interferon-β (IFN-β) release. Although IFN-β release and autophagy activation have been reported during mycobacterium infection, the mechanisms underlying remains poorly defined. Here, we demonstrated that IFN-β release increases in macrophages exposed to <italic>M. bovis</italic> and this requires the activation of the DNA sensor of interferon-γ inducible protein 204 (IFI204). Knockdown of the IFI204 in immortalized and primary murine macrophages blocked IFN-β production and autophagy marker LC3 expression. Thus, our results indicate that the IFI204 is an important sensor for innate immune responses of <italic>M. bovis</italic> infection.</p>