AUTHOR=Jiang Xiaoyuan , Yin Zhe , Yin Xiuyun , Fang Haihong , Sun Qiang , Tong Yigang , Xu Yuanhong , Zhang Defu , Feng Jiao , Chen Weijun , Song Yajun , Wang Jinglin , Chen Shuiping , Zhou Dongsheng 

TITLE=Sequencing of blaIMP-Carrying IncN2 Plasmids, and Comparative Genomics of IncN2 Plasmids Harboring Class 1 Integrons

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=7

YEAR=2017

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2017.00102

DOI=10.3389/fcimb.2017.00102

ISSN=2235-2988

ABSTRACT=<p>This work presents the complete nucleotide sequences of p0801-IMP from <italic>Klebsiella pneumoniae</italic>, p7121-IMP from <italic>K. oxytoca</italic>, and p17285-IMP from <italic>Citrobacter freundii</italic>, which are recovered from three different cases of nosocomial infection. These three plasmids represent the first fully sequenced <italic>bla</italic><sub>IMP</sub>-carrying IncN2 plasmids. Further comparative genomics analysis of all the five integron-carrying IncN2 plasmids p0801-IMP, p7121-IMP, p17285-IMP, pJIE137, and p34983-59.134kb indicates that they possess conserved IncN2 backbones with limited genetic variations with respect to gene content and organization. Four class 1 integrons (<italic>bla</italic><sub>IMP-1</sub>-carrying In1223 in p0801-IMP/p7121-IMP, <italic>bla</italic><sub>IMP-8</sub>-carrying In655 in p17285-IMP, In27 in pJIE137, and In1130 in p34983-59.134kb), two insertion sequence-based transposition units (IS<italic>Ecp1</italic>-<italic>orfRA1-14</italic> in p17285-IMP, and IS<italic>Ecp1</italic>-<italic>bla</italic><sub>CTX-<italic>M</italic>-62</sub>-Δ<italic>orf477</italic>-<italic>orfRA1-14</italic> in pJIE137), and a novel Tn<italic>1696</italic>-related transposon Tn<italic>6325</italic> carrying In1130 in p34983-59.134kb are indentified in the plasmid accessory regions. In1223 and In655 represent ancestral Tn<italic>402</italic>-associated integrons, while In27 and In1130 belong to complex class 1 integrons. The relatively small IncN2 backbones are able to integrate different mobile elements which carry various resistance markers, promoting the accumulation and spread of antimicrobial resistance genes among enterobacterial species.</p>