AUTHOR=Hua Xiaoting , Liu Lilin , Fang Youhong , Shi Qiucheng , Li Xi , Chen Qiong , Shi Keren , Jiang Yan , Zhou Hua , Yu Yunsong 

TITLE=Colistin Resistance in Acinetobacter baumannii MDR-ZJ06 Revealed by a Multiomics Approach

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=7

YEAR=2017

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2017.00045

DOI=10.3389/fcimb.2017.00045

ISSN=2235-2988

ABSTRACT=<p><italic>Acinetobacter baumannii</italic> has emerged as an important opportunistic pathogen due to its ability to acquire resistance to most currently available antibiotics. Colistin is often considered as the last line of therapy for infections caused by multidrug-resistant <italic>A. baumannii</italic> (MDRAB). However, colistin-resistant <italic>A. baumannii</italic> strain has recently been reported. To explore how multiple drug-resistant <italic>A. baumannii</italic> responded to colistin resistance, we compared the genomic, transcriptional and proteomic profile of <italic>A. baumannii</italic> MDR-ZJ06 to the induced colistin-resistant strain ZJ06-200P5-1. Genomic analysis showed that <italic>lpxC</italic> was inactivated by IS<italic>Aba1</italic> insertion, leading to LPS loss. Transcriptional analysis demonstrated that the colistin-resistant strain regulated its metabolism. Proteomic analysis suggested increased expression of the RND efflux pump system and down-regulation of FabZ and β-lactamase. These alterations were believed to be response to LPS loss. In summary, the <italic>lpxC</italic> mutation not only established colistin resistance but also altered global gene expression.</p>