AUTHOR=Pilchová Tereza , Pilet Marie-France , Cappelier Jean-Michel , Pazlarová Jarmila , Tresse Odile 

TITLE=Protective Effect of Carnobacterium spp. against Listeria monocytogenes during Host Cell Invasion Using In vitro HT29 Model

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=6

YEAR=2016

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2016.00088

DOI=10.3389/fcimb.2016.00088

ISSN=2235-2988

ABSTRACT=<p>The pathogenesis of listeriosis results mainly from the ability of <italic>Listeria monocytogenes</italic> to attach, invade, replicate and survive within various cell types in mammalian tissues. In this work, the effect of two bacteriocin-producing <italic>Carnobacterium</italic> (<italic>C. divergens</italic> V41 and <italic>C. maltaromaticum</italic> V1) and three non-bacteriocinogenic strains: (<italic>C. divergens</italic> V41C9, <italic>C. divergens</italic> 2763, and <italic>C. maltaromaticum</italic> 2762) was investigated on the reduction of <italic>L. monocytogenes</italic> Scott A plaque-forming during human infection using the HT-29 <italic>in vitro</italic> model. All <italic>Carnobacteria</italic> tested resulted in a reduction in the epithelial cell invasion caused by <italic>L. monocytogenes</italic> Scott A. To understand better the mechanism underlying the level of <italic>L. monocytogenes</italic> infection inhibition by <italic>Carnobacteria</italic>, infection assays from various pretreatments of <italic>Carnobacteria</italic> were assessed. The results revealed the influence of bacteriocin production combined with a passive mechanism of mammalian cell monolayers protection by <italic>Carnobacteria</italic>. These initial results showing a reduction in <italic>L. monocytogenes</italic> virulence on epithelial cells by <italic>Carnobacteria</italic> would be worthwhile analyzing further as a promising probiotic tool for human health.</p>