AUTHOR=Yu Qinghua , Yuan Lixia , Deng Jun , Yang Qian 

TITLE=Lactobacillus protects the integrity of intestinal epithelial barrier damaged by pathogenic bacteria

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=5

YEAR=2015

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2015.00026

DOI=10.3389/fcimb.2015.00026

ISSN=2235-2988

ABSTRACT=<p>Pathogens invade intestinal mucosal barrier through phagocytosis of antigen presenting cells (dendritic cell, microfold cells), or through the invasion into the intestinal epithelial directly. Some pathogens could damage the cell junction between epithelial cells and use the paracellular pathway as an entrance to invade. Moreover, some <italic>Lactobacillus</italic> could inhibit the adhesion of the pathogens and protect the integrity of the cell junction and mucosal barrier. This research focused on the potential therapeutic effect of <italic>Lactobacillus fructosus</italic> (<italic>L. fructosus</italic>) C2 to attenuate ETEC K88 or <italic>S. typhimurium</italic> SL1344 induced changes to mucosal barrier. The results demonstrated that treatment of polarized Caco-2 cells with <italic>L. fructosus</italic> C2 reduced the permeation of dextran, and expression of IL-8, p-ERK, and p-JNK when cells were infected with pathogenic bacteria. The findings indicated that <italic>L. fructosus</italic> C2 exerted a protective effect against the damage to the integrity of Caco-2 cells by ETEC or <italic>S. typhimurium</italic> infection.</p>