AUTHOR=Brzóstkowska Marta , Raczkowska Adrianna , Brzostek Katarzyna 

TITLE=OmpR, a response regulator of the two-component signal transduction pathway, influences inv gene expression in Yersinia enterocolitica O9

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=2

YEAR=2012

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2012.00153

DOI=10.3389/fcimb.2012.00153

ISSN=2235-2988

ABSTRACT=<p>The environmental control of invasin (<italic>inv</italic>) expression in <italic>Yersinia enterocolitica</italic> is mediated by a regulatory network composed of negative and positive regulators of <italic>inv</italic> gene transcription. Previously, we demonstrated that OmpR, a response regulator of the two-component signal transduction pathway EnvZ/OmpR, negatively regulates <italic>inv</italic> gene expression in <italic>Y. enterocolitica</italic> O9 by direct interaction with the <italic>inv</italic> promoter region. This study was undertaken to clarify the role of OmpR in the <italic>inv</italic> regulatory circuit in which RovA protein has been shown to positively regulate <italic>inv</italic> transcription. Using <italic>ompR</italic>, <italic>rovA</italic>, and <italic>ompR rovA Y. enterocolitica</italic> mutant backgrounds we showed that the inhibitory effect of OmpR on <italic>inv</italic> transcription may be observed only when RovA is present/active in <italic>Y. enterocolitica</italic> cells. To extend our research on <italic>inv</italic> regulation we examined the effect of OmpR on <italic>rovA</italic> gene expression. Analysis of <italic>rovA-lacZ</italic> transcriptional fusion in <italic>Y. enterocolitica</italic> wild-type and <italic>ompR</italic> background indicated that OmpR does not influence <italic>rovA</italic> expression. Thus, our results indicate that OmpR influences <italic>inv</italic> expression directly via binding to the <italic>inv</italic> promoter, but not through modulation of <italic>rovA</italic> expression.</p>