AUTHOR=Misir Sema , Ozer Yaman Serap , Hepokur Ceylan , Akidan Osman , Aliyazicioglu Yuksel , Enguita Francisco J. , Al Zoubi Mazhar Salim TITLE=Identification of miR-342-5p/MDM4/p53 network in acute myeloid leukemia JOURNAL=Frontiers in Cell Death VOLUME=3 YEAR=2024 URL=https://www.frontiersin.org/journals/cell-death/articles/10.3389/fceld.2024.1503241 DOI=10.3389/fceld.2024.1503241 ISSN=2813-5563 ABSTRACT=
Acute myeloid leukemia (AML) is one of the most prevalent hematological malignancies. miRNAs play roles in cancer initiation and progression in various cancer types by post-transcriptional regulation of gene expression. The aim of this study is to investigate the mechanisms in the development and progression of acute myeloid leukemia and to identify potential target genes and miRNAs by bioinformatic analysis. miRNA expression profiles were obtained from the GSE51908 dataset on the Gene Expression Omnibus (GEO). GEO2R was used to identify differentially expressed miRNAs. The diagnostic and overall survival effects of the identified miRNA were determined using ROC analysis and Kaplan-Meier curve, respectively. Putative miRNA targets were determined based on miRWalk and miRDB tools. The expression change and overall survival analysis of the identified target gene were analyzed by Gene Expression Profiling Interactive Analysis (GEPIA). Protein-protein interaction (PPI) networks of the target gene were determined using STRING and GeneMANIA. Functional enrichment analysis was performed using the DAVID program. 24 DE-miRNAs were identified, including 16 upregulated and 8 downregulated genes. miR-342-5p expression had significantly shorter survival than those in higher expression control group (