ORIGINAL RESEARCH article
Front. Cell Dev. Biol.
Sec. Molecular and Cellular Reproduction
Volume 13 - 2025 | doi: 10.3389/fcell.2025.1596421
INCREASED REPRODUCTIVE OUTCOMES AFTER OPTIMIZED SPERM PREPARATION
Provisionally accepted
Matias Gomez Elias1
Guillermina Maria Luque2
Natalia Oscoz Susinno2
Analia Guadalupe Novero3
Olinda Briski2Ines Kasparas2
Tomás Steeman3
Cintia Stival3Mariano Lavolpe4Vanina Julianelli4Marisa Geller4Martin Attie4Rita Vassena4
Dario Krapf1,3*
Mariano G Buffone2*- 1Fecundis Lab, Barcelona, Spain
- 2CONICET Institute of Biology and Experimental Medicine (IBYME), Buenos Aires, Argentina
- 3CONICET Instituto de Biología Molecular y Celular de Rosario (IBR), Rosario, Santa Fe, Argentina
- 4In vitro Buenos Aires, Buenos Aires, Argentina
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A key factor to the success of in vitro fertilization (IVF) is the preparation of human sperm, a critical step that directly impacts the efficacy of the procedure. This proof-of-concept study evaluated the effect of HyperSperm, a novel sperm preparation technique designed to enhance sperm function, on fertilization, embryo development, and pregnancy outcomes in both a mouse model and a first-in-human trial following IVF. In mice, HyperSperm treatment significantly increased hyperactivated motility (p<0.05), leading to improved fertilization and blastocyst development (p<0.05), as well as higher implantation rates (p<0.05) and larger litter sizes (p<0.05). Offspring displayed normal growth and fertility. In human sperm samples from normozoospermic men, HyperSperm exhibited a high safety profile, with motility, acrosome reaction, viability, and DNA fragmentation comparable to controls. A first-in-human, prospective, single-center, split-oocyte study in 10 couples undergoing IVF with donated oocytes demonstrated similar fertilization rates between HyperSperm and control groups (p=0.425), but significantly higher usable blastocyst rates in the HyperSperm arm (43.8% vs 67.9% , p=0.0122 ). Morphokinetic parameters were comparable between groups.These results suggest that HyperSperm enhances sperm hyperactivation, a hallmark of capacitation, leading to improved embryo development in both mice and humans. This technique represents a promising approach to optimizing sperm preparation in assisted reproduction, warranting further clinical investigation.HyperSperm enhances sperm hyperactivation, improving fertilization in mice and embryo development in both mice and humans. In a first-in-human IVF study, it significantly increased usable blastocyst rates, highlighting its potential to optimize assisted reproduction.
Keywords: Assisted Reproductive Technology, Capacitation, in vitro fertilization, implantation, Live Birth
Received: 19 Mar 2025; Accepted: 22 Apr 2025.
Copyright: © 2025 Gomez Elias, Luque, Oscoz Susinno, Novero, Briski, Kasparas, Steeman, Stival, Lavolpe, Julianelli, Geller, Attie, Vassena, Krapf and Buffone. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Dario Krapf, CONICET Instituto de Biología Molecular y Celular de Rosario (IBR), Rosario, S2000EZP, Santa Fe, Argentina
Mariano G Buffone, CONICET Institute of Biology and Experimental Medicine (IBYME), Buenos Aires, Argentina
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.